• 关键词：袁河  高通量测序  细菌群落  土地利用  方差分解

• 基金项目：国家自然科学基金（No.41661017）；鄱阳湖湿地与流域研究教育部重点实验室（江西师范大学）开放基金资助项目（No.PK2018001）

• 赵君
• 1. 江西师范大学地理与环境学院, 南昌 330022;2. 江西师范大学鄱阳湖湿地与流域研究教育部重点实验室, 南昌 330022

• 王鹏
• 1. 江西师范大学地理与环境学院, 南昌 330022;2. 江西师范大学鄱阳湖湿地与流域研究教育部重点实验室, 南昌 330022

• 徐启渝
• 1. 江西师范大学地理与环境学院, 南昌 330022;2. 江西师范大学鄱阳湖湿地与流域研究教育部重点实验室, 南昌 330022

• 张华
• 1. 江西师范大学地理与环境学院, 南昌 330022;2. 江西师范大学鄱阳湖湿地与流域研究教育部重点实验室, 南昌 330022

• 丁明军
• 1. 江西师范大学地理与环境学院, 南昌 330022;2. 江西师范大学鄱阳湖湿地与流域研究教育部重点实验室, 南昌 330022

• 摘要：于2018年8月采集袁河上游至下游的16个采样点表层水样，基于高通量测序技术分析土地利用方式下河流浮游细菌群落特征并对比土地利用与水化学指标对浮游细菌群落的影响差异.结果表明：①袁河优势浮游细菌群落为变形菌门（Proteobacteria，35.17%），放线菌门（Actinobacteria，33.99%）和拟杆菌门（Bacteroidetes，17.59%），其中Bacteroidetes丰度随流向显著降低；②受农田和建设用地增加的影响，袁河上、下游河段细菌群落多样性均随流向呈升高趋势；中游河段浮游细菌群落多样性由于湖库滞留作用明显下降；③水域和林地是土地利用方式中最佳组合因子，土地利用方式显著影响浮游细菌群落，两者相关系数为0.4797，土地利用方式对浮游细菌群落的方差解释量为31.3%；④土地利用方式对浮游细菌群落的影响大小与水化学指标相近，同时存在土地利用方式与水化学的交叉影响（方差解释量为10.9%），表明在袁河浮游细菌群落的形成中，质量效应与物种分选机制并存.本研究表明，在河流浮游细菌研究中要重视流域土地利用方式造成的外源细菌输入差异，以及土地利用方式输入的不同水化学成分对浮游细菌的分选作用.

• Abstract：In August 2018, we collected surface water samples at 16 sampling sites from the upper reaches of the Yuan River to the downstream. Based on high-throughput sequencing, we analyzed characteristics of bacterioplankton communities and distinguished the effects on bacterioplankton communities from land use and water chemicals. Our results showed that:① the dominant bacterioplankton communities in the Yuan River were Proteobacteria (35.17%), Actinobacteria (33.99%) and Bacteroidetes (17.59%), and Bacteroidetes decreased significantly in the downstream direction.②Both the abundance and richness of bacterioplankton increased in upper reaches and lower reaches with the increasing farmland and construction land area. Due to the increasing water residence time in the Jiangkou Reservoir, both the abundance and richness of bacterioplankton decreased in middle reaches. ③Waters and woodland were the subset of land use that best explained the variation in bacterioplankton communities. Land use patterns significantly influenced bacterioplankton communities with a correlation coefficient at 0.4797. A proportion of 31.3% in variance in bacterioplankon communities was explained by land use patterns. ④The effect of land use patterns on bacterioplankton community was comparable with that of water chemicals. Cross-effect on bacterioplankton community was discovered between land use and water chemicals, which accounted for 10.9% variance in bacterioplankton communities. The above results reveal that both the mechanism of mass effect and species sorting played a role in shaping bacterioplankton communities in river water. This study suggests that in the study of river planktonic bacteria, it is necessary to pay attention to the different input of exogenous bacterial from different land use in drainage basin, and the sorting effect on bacterioplankton community caused by different water chemical components input from different land use.

• Key words：the Yuan River  high-throughput sequencing  bacterial community  land use  variance partitioning analysis

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