研究报告
黄瑾璟,徐煜锋,阮静雯,邢波,叶潞洁,陈东之.两相分配体系中硅酮母粒对氯苯降解菌Delftia sp. LW26的影响[J].环境科学学报,2020,40(4):1430-1435
两相分配体系中硅酮母粒对氯苯降解菌Delftia sp. LW26的影响
- Effect of silicone masterbatch on chlorobenzene-degrading strain Delftia sp. LW26 in two-phase partitioning bioreactor
- 基金项目：国家重点研发计划课题（No.2018YFC0214103-02）；国家自然科学基金项目（No.21477116）；绍兴市科技计划项目（No.2018C30004）
- 作者
- 单位
- 黄瑾璟
- 绍兴市环境监测中心站, 绍兴 312000
- 徐煜锋
- 浙江工业大学环境学院, 杭州 310014
- 阮静雯
- 浙江工业大学环境学院, 杭州 310014
- 邢波
- 绍兴市环境监测中心站, 绍兴 312000
- 叶潞洁
- 绍兴市环境监测中心站, 绍兴 312000
- 陈东之
- 浙江工业大学环境学院, 杭州 310014
- 摘要：将硅酮母粒作为两相分配体系中的固态非水相（SNAP）来处理氯苯废气，从细胞表面疏水性（CSH）、菌体表面胞外多聚物（EPS）和电子传递体系（ETS）来探究硅酮母粒的加入对微生物特性以及活性的影响.结果表明，硅酮母粒在1 h内可捕集70%左右的氯苯，5次回收再利用的硅酮母粒在两相体系中对氯苯的去除率仍在99%以上；在较高的初始氯苯浓度下（10 mmol·L-1），两相体系中的ETS活性可达到112 mg·g-1·h-1，而在单相体系中未检测到ETS活性；两相体系中EPS量较于单相体系也有所提高，并且EPS中的蛋白质与多糖的比值是单相体系的2.34倍，这在一定程度上提高了CSH，使菌体更趋向于粘附在固/液界面，提高了传质效率；CSH总体上随硅酮母粒比例的增加而增加，且在20%时达到最大值.
- Abstract：The silicone masterbatch was used as a solid non-aqueous phase (SNAP) in a two-phase partitioning bioreactor to treat chlorobenzene-containing waste gas. The cell-surface hydrophobicity (CSH), extracellular polymeric substances (EPS) and electron transport system (ETS) were investigated, respectively, to evaluate the effect of silicone masterbatch on the characteristic and activity of the microorganism. The capture capacity of silicone masterbatch on chlorobenzene achieved approximately 70% within one hour. The removal efficiency of chlorobenzene in the bioreactor still exceeded 99% with the addition of the silicone masterbatch after five recycles. The ETS reached 112 mg·g-1·h-1 in the two-phase partitioning solution, while no ETS activity was detected in single-phase solution with the initial chlorobenzene concentration as high as 10 mmol·L-1. The amount of EPS in the two-phase partitioning bioreactor was more than that in single-phase bioreactor, and the ratio of protein to polysaccharide in the EPS in the former bioreactor was 2.34 times higher than that in the latter bioreactor. This resulted in a higher CSH which made the cells adhere to the solid/liquid interface easier and in turn improved the mass-transfer efficiency. The overall CSH showed positive correlation with the amount of silicone masterbatch addition, and reached the maximum value when 20% of silicone masterbatch was added.
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