张浩,滑紫微,牛秋红,惠丰立,董维亮,周杰,陈兆进,李娜.嗪草酮降解菌Rhodococcus sp.MA的降解特性及固定化应用[J].环境科学学报,2020,40(6):2101-2110
嗪草酮降解菌Rhodococcus sp.MA的降解特性及固定化应用
- Degradation characteristics and immobilization application of metribuzin-degrading strain Rhodococcus sp. MA
- 基金项目：河南省能源微生物资源可持续利用创新型科技团队项目；河南省高等学校重点科研项目（No.20A180020）；南阳师范学院高层次人才科研启动费资助项目（No.2019ZX015，2019ZX013）
- 张浩
- 南阳师范学院生命科学与技术学院, 南阳 473061
- 滑紫微
- 南阳师范学院生命科学与技术学院, 南阳 473061
- 牛秋红
- 南阳师范学院生命科学与技术学院, 南阳 473061
- 惠丰立
- 南阳师范学院生命科学与技术学院, 南阳 473061
- 董维亮
- 南京工业大学生物与制药工程学院, 南京 211816
- 周杰
- 南京工业大学生物与制药工程学院, 南京 211816
- 陈兆进
- 南阳师范学院农业工程学院, 南阳 473061
- 李娜
- 南阳师范学院生命科学与技术学院, 南阳 473061
- 摘要：从农田土壤中分离得到1株嗪草酮高效降解菌株，命名为MA，该菌5 d对50 mg·L-1嗪草酮的降解率达到74.8%，最适反应温度和pH分别为30℃和7.0.根据表型与生理生化特征及16S rRNA序列分析，将其鉴定为Rhodococcus sp.MA.利用气相色谱-质谱联用（GC-MS）鉴定菌株MA降解嗪草酮的中间产物为脱氨基嗪草酮.以海藻酸钠为包埋剂，经正交试验表明，3%海藻酸钠、2% CaCl2、包埋比8：1和固定时间6 h条件下，菌株对嗪草酮的降解效率最高，为71.2%.将固定化菌体细胞用于修复实验，结果表明，未固定化处理的菌液对湖水及池塘水中嗪草酮（15 mg·L-1）的去除率分别为72.1%和69.9%；固定化处理后可提高对嗪草酮的去除率，分别达到87.3%和82.6%，具有良好的应用前景.
- Abstract：Strain MA was isolated from farmland soil, which was capable of degrading 74.8% of 50 mg·L-1 within 5 d. The optimum degradation temperature and pH were 30℃ and 7.0, respectively. Strain MA was identified as Rhodococcus sp. MA based on morphological observation, physiological characteristics and its 16S rRNA gene sequence. The degradation product of metribuzin was identified as deamino-metribuzin by GC-MS. The orthogonal test results showed that the degradation efficiency of metribuzin was the highest (71.2%) when using 3% sodium alginate as the embedding agent, 2% CaCl2, embedding ratio of 8:1 and fixed time of 6 h. When unimmobilized bacteria were used in the bioremediation experiment, the removal rates of metribuzin in lake and pond water (15 mg·L-1) were 72.1% and 69.9%,respectively. The removal rates were increased to 87.3% and 82.6%, respectively with immobilized bacteria, suggesting promising application prospect in bioremediation area.
