• 关键词：菇渣  蚯蚓生物转化  纤维素  木质素

• 基金项目：辽宁省大学生创新创业训练资助项目（No.201811430060）；国家自然科学基金青年基金资助项目（No.41701300，21702086）；辽宁省自然科学基金指导计划资助项目（No.201601338）

• 史雅静
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 徐明
• 本钢板材股份有限公司, 本溪 117000

• 王振宇
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 庞月
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 张改琴
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 贾媛媛
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 李秀超
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 方永俊
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 韩雪松
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 赵秋伶
• 辽宁科技学院生物医药与化学工程学院, 辽宁省生物医药与化学工程重点实验室, 本溪 117004

• 摘要：为了探索农业废弃香菇菇渣"减量化、无害化、资源化"循环化利用的有效途径.本实验按照不同碳氮比设置菇渣混合基质，接种蚯蚓（Eisenia foetida）对其进行生物转化.实验培养56 d，每14 d取样，分别测定了总氮、总有机碳、木质素和纤维素含量，脲酶、蔗糖酶、纤维素酶和多酚氧化酶活性变化情况，以及菇渣生物转化前后的结构变化情况（扫描电子显微镜SEM）和生物转化产物浸提液的发芽率等指标.结果显示：在蚯蚓生物转化过程中，各处理组总氮、总有机碳和木质纤维素含量与处理前相比均有不同程度的降低，其中，碳氮比为25的处理组纤维素和木质素的降解率最高，分别为50.06%和77.01%；各个处理组中脲酶、蔗糖酶、纤维素酶和多酚氧化酶活性呈不同程度的变化趋势，其中，碳氮比为25的处理组纤维素酶活性增加最多，增加了81.25%；扫描电镜结果显示，蚯蚓生物转化可以将菌渣碎片化；另外，蚯蚓堆肥产物浸提液可以提高黑麦草发芽率.综上，推测碳氮比为25的蚯蚓生物转化体系更有利于菇渣基质处理.

• Abstract：In order to explore the effective method of recycle utilization of mushroom (Shii-take) residue, the mixtures of mushroom residue with different carbon and nitrogen (C/N) ratios were prepared, and the earthworms (Eisenia foetida) were inoculated for biotransformation. Briefly, the earthworms were inoculated into the mixtures of mushroom residue with different C/N ratios and cultivated for 56 days, and the samples were collected every 14 days. Then, the contents of total nitrogen, organic carbon, lignin and cellulose in samples were determined; the activities of urease, invertase, cellulase and polyphenol oxidase in samples were examined; the structure of the mushroom residue was analyzed by scanning electron microscopy (SEM); and the germination rates of ryegrass treated with the extract derived from above mixtures were also examined. Consequently, after being treated with earthworms, the contents of total nitrogen, organic carbon, lignin and cellulose in samples of all treated groups had significant decreases. Especially in the treated group with C/N ratios 25, in which the degradation rates of cellulose (50.06%) and lignin (77.01%) reached to the lowest level in all treated groups. Simultaneously, the activities of urease, invertase, cellulase and polyphenol oxidase in samples of all earthworms-treated groups were enhanced significantly, and also in the treated group with C/N ratios 25, the activities of enzymes reach to the highest level (up to 81.25%) in all treated groups. Additionally, the results of SEM demonstrated that the earthworms can promote the fragmentation of mushroom residue, also the extract derived from earthworms treated mixtures can improve the germination rates of ryegrass. Conclusively, it reasonably speculates that earthworms derived biotransformation contribute to the treatment of mushroom residue, and the mixture with C/N ratios 25 is optimal for earthworms derived biotransformation.

• Key words：mushroom residue  earthworm biotransformation  cellulose  lignin

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