滕晓强,陆宏峰,郭心月,高畅,罗钦方,张瑜,洪华嫦,孙洪杰.二氯苯醌对斑马鱼胚胎发育及超氧化物歧化酶的影响[J].环境科学学报,2020,40(7):2659-2664
二氯苯醌对斑马鱼胚胎发育及超氧化物歧化酶的影响
- The effect of 2,6-DCBQ on the development and superoxide dismutase of zebrafish embryo
- 基金项目：国家自然科学基金（No.21707123）；浙江省公益应用项目（No.LGF19B070007）
- 滕晓强
- 浙江师范大学地理与环境科学学院, 金华 321004
- 陆宏峰
- 浙江师范大学地理与环境科学学院, 金华 321004
- 郭心月
- 浙江师范大学地理与环境科学学院, 金华 321004
- 高畅
- 浙江师范大学地理与环境科学学院, 金华 321004
- 罗钦方
- 浙江师范大学地理与环境科学学院, 金华 321004
- 张瑜
- 浙江师范大学地理与环境科学学院, 金华 321004
- 洪华嫦
- 浙江师范大学地理与环境科学学院, 金华 321004
- 孙洪杰
- 浙江师范大学地理与环境科学学院, 金华 321004
- 摘要：2，6-二氯-1，4-苯醌（2，6-dichloro-1，4-benzoquinone，2，6-DCBQ）作为一种新型消毒副产物，因其高检出率及高含量的特点而备受人们关注.本研究以斑马鱼胚胎为实验材料探究了2，6-DCBQ的发育毒性.结果发现：斑马鱼胚胎在8~24 hpf（器官发育形成时期）对2，6-DCBQ暴露最为敏感，死亡率最高；2，6-DCBQ安全浓度为27.15 μg·L-1，属于极度危险外源化学物.在本实验中，2，6-DCBQ对斑马鱼的生长没有显著影响，但高浓度（100和150 μg·L-1）2，6-DCBQ会显著增加斑马鱼胚胎及仔鱼的畸形率.在实验过程中还发现，高浓度（≥ 80 μg·L-1）2，6-DCBQ会明显抑制斑马鱼胚胎的心率.此外，≥ 30 μg·L-1 2，6-DCBQ可提高超氧化物歧化酶（Superoxide dismutase，SOD）的活性，并影响Cu/ZnSOD和MnSOD的正常转录.本研究较全面地探究了2，6-DCBQ对斑马鱼早期发育阶段的发育毒性，可为2，6-DCBQ的毒性研究提供可靠证据.
- Abstract：2,6-dichloro-1,4-benzoquinone (2,6-DCBQ), as an emerging disinfection by-products, has attracted much attentions due to frequently detected and high levels. In this study, zebrafish embryo was employed to explore the toxic effect of 2,6-DCBQ on the developmental performance of zebrafish embryo. Our results clearly indicated that zebrafish embryo at 8~24 hpf was most sensitive to 2,6-DCBQ, and more embryos died at 8~24 hpf. The safe concentrations of 2,6-DCBQ was 27.15 μg·L-1, and thus 2,6-DCBQ is classified as the extremely dangerous pollutant. In the present study, 2,6-DCBQ had no significant effect on zebrafish growth, whereas higher concentrations (100 and 150 μg·L-1) of 2,6-DCBQ obviously inhibited zebrafish embryo heart beating. Besides, in this study, over 30 μg·L-1 of 2,6-DCBQ significantly elevated superoxide dismutase (SOD) activity and disrupted the transcriptional levels of Cu/ZnSOD and MnSOD. Our results comprehensively demonstrate the toxic effect of 2,6-DCBQ on developmental performance of zebrafish embryos, providing reliable evidence for 2,6-DCBQ toxicity.
