钟云浩,何宇哲,宋朝红,彭星星,贾晓珊.不同碳源模式下酵母菌PNY2013同步脱氮除磷的应用研究[J].环境科学学报,2020,40(9):3211-3223
不同碳源模式下酵母菌PNY2013同步脱氮除磷的应用研究
- Application of simultaneous nitrogen and phosphorus removal by yeast PNY2013 under different carbon source modes
- 基金项目：国家自然科学基金（No.20183800041030649）；广东省科技计划粤港联合创新项目（No.20163800042410510）；广东省科技厅项目（No.20183800042050640）
- 钟云浩
- 1. 中山大学环境科学与工程学院, 广州 510275;2. 广东省环境污染控制与修复技术重点实验室, 广州 510275
- 何宇哲
- 1. 中山大学环境科学与工程学院, 广州 510275;2. 广东省环境污染控制与修复技术重点实验室, 广州 510275
- 宋朝红
- 1. 中山大学环境科学与工程学院, 广州 510275;2. 广东省环境污染控制与修复技术重点实验室, 广州 510275
- 彭星星
- 1. 中山大学环境科学与工程学院, 广州 510275;2. 广东省环境污染控制与修复技术重点实验室, 广州 510275
- 贾晓珊
- 1. 中山大学环境科学与工程学院, 广州 510275;2. 广东省环境污染控制与修复技术重点实验室, 广州 510275
- 摘要：针对首次分离得到的一株具有同步脱氮除磷新功能的热带假丝酵母（Candida tropicalis） PNY2013，通过生理及动力学特征，连续流运行操作及其在含糖类工业废水中的应用3个环节，探讨了不同碳源模式下PNY2013同步脱氮除磷的特性.结果表明：PNY2013以葡萄糖、乙醇及乙酸为唯一碳源时均生长良好，其最大比增长速率μmax分别为0.1327、0.1252及0.1115 h-1，其同步脱氮除磷率分别可达100%、80%、100%（NH4+-N）及93%、95%、98%（PO43--P）.3种碳源下PNY2013同步脱氮除磷的最佳条件基本接近为：温度30℃，pH=8.0，溶解氧0~2 mg·L-1，C/N=200∶5左右.PNY2013同步脱氮除磷的长期连续运行条件下的实验进一步表明，以葡萄糖为碳源条件下，进水NH4+-N及PO43--P浓度分别达400及80 mg·L-1时，两者去除率均接近100%.与这种超强能力相比，以乙醇及乙酸为碳源条件下，进水NH4+-N及PO43--P浓度分别达100及20 mg·L-1时，两者的去除率也可达60%~80%（NH4+-N）及40%（PO43--P），显示出相当的同步脱氮除磷能力.在以模拟制糖废水、淀粉加工废水、啤酒废水、味精废水这4种典型含糖工业废水为碳源条件下，除淀粉加工废水外PNY2013均能有效去除COD、NH4+-N和PO43--P，其中，制糖、啤酒、制药废水中的COD去除率分别可达40%、89%、96%，NH4+-N去除率分别为85%、94%、76%，PO43--P去除率均为90%.即使在40000 mg·L-1（制糖）及12500 mg·L-1（啤酒）的高COD条件下，PNY2013也均具有稳定的NH4+-N和PO43--P去除效果，显示出良好的同步脱氮除磷应用前景.
- Abstract：The present study reported a purified yeast strain of PNY2013, which is able to simultaneously remove the nitrogen and phosphorus for the first time. Based on the study of its physiological and dynamic characteristics under continuous flow reactor and its application on sugar-containing industrial wastewater, characteristics of PNY2013 simultaneous nitrogen and phosphorus removal under different carbon source modes were discussed. The results show that PNY2013 grows well with glucose, ethanol, and acetic acid as the sole carbon sources, and its maximum specific growth rates μmax are 0.1327, 0.1252, and 0.1115 h-1, respectively. And the simultaneous removal rates of nitrogen and phosphorus with different carbon source are:100%, 80%, 100%(NH4+-N) and 93%, 95%, 98%(PO43--P), respectively. Under the three carbon sources, the optimal conditions for simultaneous nitrogen and phosphorus removal in PNY2013 are basically close to:temperature=30℃, pH=8.0, DO=0~2 mg·L-1, C/N=200:5. With glucose as the carbon source, the concentrations of influent NH4+-N and PO43--P are 400 and 80 mg·L-1, respectively, and the removal rates of both are close to 100%. Compared with this super capacity, when acetic acid was used as a carbon source, the removal rates of NH4+-N and PO43--P at 100 and 20 mg·L-1 can be 60%~80% (NH4+-N) and 40% (PO43--P), respectively. These results show considerable simultaneous nitrogen and phosphorus removal capabilities. With simulating sugar wastewater, starch wastewater, beer wastewater, monosodium glutamate wastewater as four carbon sources, PNY2013 can effectively remove COD, NH4+-N and PO43--P under all conditions except starch wastewater. The removal rates of sugar, beer, and MSG can reach 40%, 89%, 96% (COD); 85%, 94%, 76%(NH4+-N); 90%, 90%, 90% (PO43--P), respectively. Even under high COD value of 40000 mg·L-1 (sugar) and 12500 (beer) mg·L-1, PNY2013 has stable NH4+-N and PO43--P removal efficiency, showing good application prospects for simultaneous nitrogen and phosphorus removal.
