• 关键词：互花米草入侵  河口湿地  细菌  多样性  群落结构

• 基金项目：国家自然科学基金（No.41571287）；福建省高校杰出青年科研人才培育计划2017

• 林少颖
• 福建师范大学地理研究所, 福州 350007

• 陈桂香
• 福建师范大学地理研究所, 福州 350007

• 刘旭阳
• 福建师范大学地理研究所, 福州 350007

• 金强
• 福建师范大学地理研究所, 福州 350007

• 王晓彤
• 福建师范大学生命科学学院, 福州 350117

• 曾从盛
• 1. 福建师范大学地理研究所, 福州 350007;2. 福建师范大学湿润亚热带生态地理过程教育部重点实验室, 福州 350007

• 王维奇
• 1. 福建师范大学地理研究所, 福州 350007;2. 福建师范大学湿润亚热带生态地理过程教育部重点实验室, 福州 350007

• 摘要：本研究以闽江口秋茄湿地、漳江口白骨壤湿地和闽江口芦苇湿地为研究地点，采集土壤样品并通过高通量测序分析了土壤细菌的群落结构及其多样性，以此探讨互花米草入侵对河口湿地土壤细菌群落结构的影响.结果表明：①互花米草入侵河口湿地后，改变了湿地土壤细菌种类的相对丰度，且入侵不同的湿地群落，细菌优势菌属及相对丰度具有差异；②互花米草入侵秋茄和芦苇湿地后使土壤细菌多样性升高，互花米草入侵白骨壤湿地后使土壤细菌多样性降低；③互花米草入侵并未明显改变细菌门水平的群落组成，湿地土壤主要的细菌门为变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）、绿弯菌门（Chloroflexi）、酸杆菌门（Acidobacteria）、拟杆菌门（Bacteroidetes）；④互花米草入侵改变了湿地土壤细菌的优势菌属，使白骨壤湿地土壤Ruminococcus_2、Faecalibacterium的相对丰度降低，同时白骨壤湿地土壤出现了Thiohalophilus、Hallea等优势菌属；⑤环境因子和活性碳组分能够影响湿地土壤中细菌的相对丰度，细菌Thiohalophilus的相对丰度与湿地土壤盐度呈显著正相关（p<0.05），Sideroxydans的相对丰度与湿地土壤pH呈显著负相关（p<0.01），Ruminococcus_2的相对丰度与土壤易分解有机碳（LOC）呈显著负相关（p<0.01）；Blautia、Bacteroidess的相对丰度与土壤可溶性有机碳（DOC）呈显著正相关（p<0.01）.本研究可为深入研究互花米草入侵机制及生态影响提供重要科学依据.

• Abstract：In this study, we collected soil samples from three sites (i.e., Kandelia candel wetland in Minjiang River Estuary, the Avicennia marina wetland in Zhangjiang River Estuary, and the Phragmites australis wetland in Minjiang River Estuary), and soil bacterial community structure and diversity was measured by high-throughput sequencing in the native and Spartina alterniflora invasion sites. The results showed: ① The relative abundance of soil bacterial groups were changed after Spartina alterniflora invasion in estuarine wetland. Besides, the relative abundance of dominant bacterial genera was also different in various wetland. ② Soil bacterial diversity was increased by Spartina alterniflora invasion in the Kandelia candel and Phragmites australis wetland, while soil bacterial diversity was increased in the Avicennia marina wetland. ③ Bacteria community composition was not significantly changed by Spartina alterniflora invasion at phylum level. Bacterial phyla were Proteobacteria, Firmicutes, Chloroflexi, Acidobacteria and Bacteroidetes in these wetland soils. ④ Bacterial community composition was altered by Spartina alterniflora invasion at genus level, and the relative abundance of Ruminococcus_2 and Faecalibacterium was decreased, however, dominant genera such as Thiohalophilus and Hallea was increased in Avicennia marina wetland. ⑤ The relative abundance of bacteria was influenced by the environmental factors and active carbon component in wetland soils. The relative abundance of Thiohalophilus was significantly and positively correlated with the soil salinity (p<0.05), and the relative abundance of Sideroxydans was significantly and negatively correlated with soil pH (p<0.01). There was a significant and negative correlation between the relative abundance of Ruminococcus_2 and labile organic carbon (p<0.01). The relative abundance of Blautia and Bacteroides were significantly and positively correlated with soil dissolved organic carbon (p<0.01). The study could provide important scientific basis for further study of Spartina alterniflora invasion mechanism and the related ecological impact.

• Key words：Spartina alterniflora invasion  estuarine wetland  bacteria  diversity  community structure

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