• 关键词：河岸过滤  氨氮削减  细菌  氨氧化微生物  群落结构

• 基金项目：国家自然科学基金项目（No.51820105011）

• 赵健
• 清华大学环境学院, 清华大学水质与水生态研究中心, 北京 100084

• 王巧娟
• 中国科学院生态环境研究中心, 饮用水科学与技术重点实验室, 北京 100085

• 柏耀辉
• 中国科学院生态环境研究中心, 饮用水科学与技术重点实验室, 北京 100085

• 齐维晓
• 清华大学环境学院, 清华大学水质与水生态研究中心, 北京 100084

• 刘会娟
• 清华大学环境学院, 清华大学水质与水生态研究中心, 北京 100084

• 曲久辉
• 1. 清华大学环境学院, 清华大学水质与水生态研究中心, 北京 100084;2. 中国科学院生态环境研究中心, 饮用水科学与技术重点实验室, 北京 100085

• 摘要：通过小试滤柱模拟河岸过滤，结合荧光定量PCR和高通量测序等手段研究了河岸过滤对氨氮的削减效能和进水氨氮浓度对细菌与氨氧化微生物的影响.结果表明，低浓度（0.2 mg·L^-1）氨氮未被削减，较高浓度（1.0 mg·L^-1和2.0 mg·L^-1）氨氮的削减率可达70%.进水处细菌丰度随氨氮浓度升高而增加，细菌群落组成随氨氮浓度升高发生规律性变化.出水处氨氧化古菌（Ammonia oxidizing archaea，AOA）丰度与氨氮浓度呈负相关，氨氧化细菌（Ammonia oxidizing bacteria，AOB）丰度与氨氮浓度无显著相关关系；进水处AOA-α多样性与氨氮浓度呈正相关，但AOB-α多样性与氨氮浓度呈负相关；进水处AOA群落组成受氨氮浓度影响，而进、出水处AOB群落组成均受氨氮浓度影响.可见，进水氨氮浓度会影响河岸过滤对氨氮的削减效能，还会影响细菌、氨氧化微生物的丰度及群落结构，AOB对氨氮浓度的变化比AOA更敏感.

• Abstract：Ammonium nitrogen removal during riverbank filtration (RBF) and the effects of its concentration on bacteria and ammonia oxidizing microorganisms were explored in laboratory-scale column study using real-time PCR and high-throughput sequencing. Results showed that ammonium nitrogen could not be removed at its concentration of 0.2 mg·L^-1, while its removal efficiency could be up to 70% at initial concentration of 1.0 mg·L^-1 and 2.0 mg·L^-1. Bacterial abundance of inlets of columns increased with ammonium nitrogen concentration. Bacterial community composition of inlets changed regularly with increasing ammonium nitrogen concentration. Ammonia oxidizing archaea (AOA) abundance of outlets were negatively correlated with ammonium concentration, but no significant correlation was found between Ammonia oxidizing bacteria (AOB) abundance and ammonium nitrogen concentration; Ammonium nitrogen concentration was positively correlated with AOA-α diversity of inlets, but negatively correlated with AOB-α diversity of inlets; AOA community composition of inlets were affected by ammonium nitrogen concentration, while AOB community composition of inlets and outlets were both affected by ammonium nitrogen concentration. Overall, ammonium nitrogen concentration affects its removal efficiency during RBF and it also affects abundance and community composition of bacteria and ammonia-oxidizing microorganisms. AOB are more sensitive to the changes in ammonium nitrogen concentration than AOA.

• Key words：riverbank filtration  ammonium nitrogen removal  bacteria  ammonia-oxidizing microorganisms  community composition

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