• 关键词：琼氏不动杆菌  异养硝化-好氧反硝化  动力学  中间产物

• 基金项目：温州市科技计划项目（No.W20170009）

• 刘方剑
• 温州大学生命与环境科学学院, 温州 325000

• 杨海龙
• 温州大学生命与环境科学学院, 温州 325000

• 周化斌
• 温州大学生命与环境科学学院, 温州 325000

• 摘要：异养硝化-好氧反硝化细菌Acinetobacter junii WZ17脱氮效果良好，为确定其脱氮特性及动力学过程，利用“样条插值法”研究了菌株生长阶段，并采用Logistic模型和修正的Gompertz模型对菌株生长及氮素去除过程进行拟合，结合反硝化过程中间产物，分析菌株脱氮途径.结果显示，菌株WZ17以NH₄⁺-N、NO₃^--N和NO₂^--N唯一氮源时，生长适应期分别为2.89、3.13和3.13 h，最大去除速率分别为8.47、5.76和5.18 mg·L^-1·h^-1，生长和底物去除过程分别符合Logistic模型（R²>0.9）和修正的Gompertz模型（R²>0.9）.硝化过程中，NO₃^--N和NO₂^--N的积累量仅为0.13和0.14 mg·L^-1，反硝化过程中，NO₂^--N的积累量为1.55 mg·L^-1.“样条插值法”的运用可以准确地划分菌株WZ17的生长阶段，菌株WZ17对NH₄⁺-N、NO₃^--N和NO₂^--N均具有较好的去除效果，反硝化途径为NO₃^--N→NO₂^--N→N_xO_y.

• Abstract：Acinetobacter junii WZ17, a heterotrophic nitrification-aerobic denitrification bacterium, has good nitrogen removal efficiency. To explore its nitrogen removal characteristic and kinetics, the spline interpolation method was used to divide the growth stage of the strain, and the growth and nitrogen removal kinetics were fitted by logistic model and the modified Gompertz model, respectively. Furthermore, the nitrogen metabolic pathway of strain WZ17 was elucidated by analyzing the intermediates. The results showed that the lag phase of the strain was 2.89, 3.13 and 3.13 h, and the maximum nitrogen removal rate was 8.47, 5.76 and 5.18 mg·L^-1·h^-1 when NH₄⁺-N, NO₃^--N and NO₂^--N was used as the sole nitrogen source, respectively. The growth and substrate removal processes well fitted by the logistic model (R² > 0.9) and the modified Gompertz model (R²> 0.9), respectively. The accumulation of NO₃^--N and NO₂^--N was only 0.13 and 0.14 mg·L^-1 during nitrification, respectively, while the accumulation of NO₂^--N was 1.55 mg·L^-1 during denitrification. The growth stage of strain WZ17 is accurately divided by spline interpolation method. Strain WZ17 can efficiently remove NH₄⁺-N, NO₃^--N and NO₂^--N, and the denitrification pathway is NO₃^--N→NO₂^--N→N_xO_y.

• Key words：Acinetobacter junii WZ17  heterotrophic nitrification-aerobic denitrification  kinetic  intermediates

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