宋亚朋,宫徽,苑泉,常风民,王建兵,王凯军.不同形态厌氧氨氧化菌处理磁分离出水脱氮性能及微生物群落结构变化分析[J].环境科学学报,2021,41(1):70-82
不同形态厌氧氨氧化菌处理磁分离出水脱氮性能及微生物群落结构变化分析
- Nitrogen removal performance through different AnAOB processes treating wastewater pretreated by magnetic separation and microbial community structure shifting analysis
- 基金项目：国家水体污染控制与治理科技重大专项（No.2017ZX07102，2017ZX07102-002）
- 宋亚朋
- 1. 清华大学, 环境学院, 北京 100084;2. 中国矿业大学(北京), 化学与环境工程学院, 北京 100083
- 王建兵
- 中国矿业大学(北京), 化学与环境工程学院, 北京 100083
- 摘要：采用两种不同形态（固定生物膜和颗粒态）的厌氧氨氧化菌（AnAOB），考察了其对磁分离出水的脱氮性能、氮负荷的差异，同时从分子生物学的角度分析了微生物群落结构的变化.结果表明：采用自模拟污水，35℃恒温、不同水力停留时间（HRT）条件下，两个反应器对NH4+-N和NO2--N的去除率均大于90%.此外，反应器内的微生物群落结构也发生改变，固定生物膜和颗粒反应器中Candidatus Kuenenia菌属消失，Candidatus_Brocadia、Candidatus_Jettenia成为体系厌氧氨氧化优势菌属，相对丰度分别上升至0.89%、0.63%（固定生物膜）和8.79%、2.92%（颗粒）.采用磁分离出水，随着HRT的降低，两种形态的厌氧氨氧化菌对NH4+-N和NO2--N去除率均在80%以上.反应器中厌氧氨氧化菌Candidatus_Brocadia、Candidatus_Jettenia的相对丰度明显下降，最终稳定维持在0.7%左右，并伴随异养菌的出现.
- Abstract：Two different anaerobic ammonia oxidation bacteria (AnAOB) processes (A: fixed biofilm process, B: granular process), which treated wastewater pretreated by magnetic separation, were compared. The performance of nitrogen removal and the nitrogen loading were investigated in this study. At the same time, the microbial community structure shift was also analyzed. The results showed that when treating simulated wastewater under different HRTs (5.4 h, 2.7 h and 2.1 h), both of NH4+-N and NO2--N removal rates were more than 90% at the constant temperature of 35 ℃. The microbial community analysis of both reactors revealed that the Candidatus Kuenenia bacteria disappeared, while Candidatus Brocadia and Candidatus Jettenia became the dominant AnAOB bacteria with relative abundance increasing to 0.89%, 0.63% (fixed biofilm) and 8.79%, 2.92% (granular), respectively. When treating wastewater pretreated by magnetic separation, the removal rates of NH4+-N and NO2--N at different HRTs (8 h, 4 h, 2 h and 1 h) were maintained above 80% for both reactors. The relative abundance of Candidatus Brocadia and Candidatus Jettenia were decreased (stable at about 0.7%) with the heterotrophic bacteria showing up.
