庞小可,许继飞,张秋萍,秦帅,朱文博,赵吉.添加四环素和重金属锌对牛粪厌氧消化性能及四环素类抗性基因丰度的影响[J].环境科学学报,2021,41(4):1487-1495
添加四环素和重金属锌对牛粪厌氧消化性能及四环素类抗性基因丰度的影响
- Effects of tetracycline and Zn on anaerobic digestion of dairy manure and the variations in tetracycline resistance genes
- 基金项目：国家自然科学基金（No.51768048，51641805）；内蒙古自治区科技计划资助项目（No.2020GG0081）；内蒙古自然科学基金（No.2020MS05003）
- 庞小可
- 内蒙古大学生态与环境学院, 呼和浩特 010021
- 许继飞
- 1. 内蒙古环境污染控制与废物资源化重点实验室, 呼和浩特 010021;2. 内蒙古大学生态与环境学院, 呼和浩特 010021
- 张秋萍
- 内蒙古大学生态与环境学院, 呼和浩特 010021
- 秦帅
- 内蒙古大学生态与环境学院, 呼和浩特 010021
- 朱文博
- 内蒙古大学生态与环境学院, 呼和浩特 010021
- 赵吉
- 1. 内蒙古环境污染控制与废物资源化重点实验室, 呼和浩特 010021;2. 内蒙古大学生态与环境学院, 呼和浩特 010021
- 摘要：为探究奶牛粪便中抗生素和重金属残留对厌氧消化和抗生素抗性基因的影响，本研究通过添加四环素（TC）、金属锌（Zn）与共同添加TC和Zn，分析了厌氧消化性能及四环素类抗性基因（tetC、tetG、tetO、tetT、tetW和tetX）和I类整合酶基因（intI1）的变化.结果表明，添加TC或Zn会抑制厌氧消化产气，二者共同添加抑制更显著（p<0.05）.添加TC或Zn对理化因子（TOC、SCOD、NH4+-N和pH值）无显著影响，对抗性基因的丰度总量具有显著影响（p<0.01）.消化后对照组抗性基因相对丰度总量降低了0.58 log，添加TC增加了0.19 log，添加Zn降低了0.39 log，添加TC+Zn增加达0.86 log.分别添加TC和Zn，会促进intI1丰度升高，并抑制消化对tetC、tetT、tetW和tetX丰度的消减，共同添加TC和Zn时抑制更明显.粪便中TC与Zn共同残留对厌氧消化消减ARGs的抑制作用远大于其中任一因子带来的抑制作用，这是需要关注的一大重要问题.
- Abstract：To explore the effects of the residual antibiotics and heavy metals in dairy manure on anaerobic digestion and variations in antibiotic resistance genes, tetracycline (TC), Zn, as well as TC and Zn were respectively added into the dairy manure. The anaerobic digestion performance and the variations in tetracycline resistance genes (tetC, tetG, tetO, tetT, tetW and tetX) and intI1 were analyzed. The results showed that TC or Zn inhibited the biogas production, and the inhibition was more significant when TC and Zn were both added (p<0.05). Adding TC or Zn had no significant influence on the physicochemical properties (TOC, SCOD, NH4+-N and pH values), whereas it had a significant influence on the total abundance of tetracycline resistance genes (p<0.01). The total relative abundance of the gene targets decreased by 0.58 log in the control group, but only 0.39 log for Zn group, and increased by 0.19 and 0.86 log when TC and TC+Zn were respectively added. Adding TC or Zn contributed to the increase of the abundance of intI1 and inhibited the abundance reductions of tetC、tetT、tetW and tetX during anaerobic digestion. The co-residue of TC and Zn in dairy manure had an inhibitory effect on the reduction of ARGs during anaerobic digestion, which was far greater than the inhibitory effect brought by any of these factors. This is an important issue that needs attention.
