彭玲,王海月,毛念佳,任洪强,耿金菊,许柯.MnO2强化厌氧氨氧化工艺启动及其微生物机理研究[J].环境科学学报,2021,41(4):1283-1292
MnO2强化厌氧氨氧化工艺启动及其微生物机理研究
- Cultivation of anammox sludge in an EGSB reactor with MnO2 powder addition
- 基金项目：国家水体污染控制与治理科技重大专项（No.2017ZX07202003）
- 彭玲
- 南京大学环境学院, 污染控制与资源再利用国家重点实验室, 南京 210023
- 王海月
- 南京大学环境学院, 污染控制与资源再利用国家重点实验室, 南京 210023
- 毛念佳
- 南京大学环境学院, 污染控制与资源再利用国家重点实验室, 南京 210023
- 任洪强
- 南京大学环境学院, 污染控制与资源再利用国家重点实验室, 南京 210023
- 耿金菊
- 南京大学环境学院, 污染控制与资源再利用国家重点实验室, 南京 210023
- 许柯
- 南京大学环境学院, 污染控制与资源再利用国家重点实验室, 南京 210023
- 摘要：通过对照试验考察了添加MnO2对厌氧氨氧化污泥驯化和培养的影响，并采用16S rRNA基因高通量测序技术和定量PCR分析其机理.结果表明，添加MnO2粉末能够显著提高容积氮负荷及运行性能，其脱氮速率高达848.04 mg·L-1·d-1，几乎是对照组反应器的2倍.16S rRNA基因高通量测序技术和定量PCR结果表明，添加MnO2粉末促进了典型厌氧氨氧化菌Candidate Brocadia的富集，厌氧氨氧化菌16S rRNA基因和功能基因hzsB的丰度显著提高.
- Abstract：In this study, the effect of adding MnO2 on the acclimation and cultivation of anammox sludge was investigated through controlled experiments. High-throughput 16S rRNA gene sequencing and qPCR were used to clarify the mechanism. The results showed that the addition of MnO2 powder could increase the volume nitrogen load and performance, and the nitrogen removal efficiency reach up to 848.04 mg·L-1·d-1, which was almost twice as high as that of the control reactor. Moreover, the results of high-throughput 16S rRNA gene sequencing and qPCR revealed that the addition of MnO2 powder promoted the enrichment of typical anaerobic ammonium oxidation bacteria Candidate Brocadia, and the abundance of 16S rRNA gene and functional gene hzsB of anammox were significantly improved.
