研究报告
王世豪,石明浩,刘苏.顺序暴露场景下四环素和砷对斑马鱼的联合毒性效应[J].环境科学学报,2020,40(12):4590-4597
顺序暴露场景下四环素和砷对斑马鱼的联合毒性效应
- Combined toxicity of tetracycline and arsenic on zebrafish in sequential exposure scenarios
- 基金项目：江苏省自然科学基金青年基金项目（No.BK20170633）；中国药科大学基本科研业务费重点项目（No.2632020ZD12）；中国药科大学大学生创新创业训练计划项目（No.201910316217）
- 作者
- 单位
- 王世豪
- 中国药科大学工学院环境科学教研室, 南京 211198
- 石明浩
- 中国药科大学工学院环境科学教研室, 南京 211198
- 刘苏
- 中国药科大学工学院环境科学教研室, 南京 211198
- 摘要：在一些不连续生产的工业活动、畜禽养殖等间歇性排放废水的场景中，水环境中的盐酸四环素（Tetracycline，TET）和砷（Arsenic，As）可能会产生区别于单一或复合暴露的顺序暴露场景，从而导致复杂的生物毒性.本研究通过分析模式生物斑马鱼的表型数据（肥满度）、病理损伤（H&E染色实验）及氧化损伤（丙二醛和还原型谷胱甘肽含量），探究顺序暴露方式下TET和As（Ⅲ）的联合毒性效应.结果表明：50 μg·L-1 TET可造成肝脏和肠道的病理学损伤，并进一步诱导氧化损伤.100 μg·L-1 As（Ⅲ）可造成肝脏炎性细胞浸润及肝脏和肠道的氧化损伤.连续暴露TET和As（Ⅲ）导致斑马鱼肥满度降低，且氧化损伤明显加剧，这可能与TET损伤了抗氧化防御系统有关.值得注意的是，TET暴露后设置2周的恢复期，可减轻As（Ⅲ）对斑马鱼肝脏和肠道的损伤，其机理可能与生物对污染物的适应及交叉抗性相关.本研究的实验结果可为评估TET和As（Ⅲ）的联合毒性效应提供新的视角.
- Abstract：In cases of intermittent wastewater discharge, such as industrial activities with discontinuous production and livestock and poultry breeding, Tetracycline (TET) and Arsenic (As) may not coexist in waters at the same time, resulting in sequential exposure which is different from single or combined exposure, leading to complex toxic effects on organisms. In this study, zebrafish was selected, and the possible sequential exposure in real scenarios was adopted to explore the combined toxic effects of TET and As (Ⅲ). The condition of zebrafish was measured. The pathological damage of liver and intestine were observed by hematoxylin-eosin staining. The oxidative damage was determined by measuring the contents of malondialdehyde (MDA) and glutathione (GSH). The results showed that two weeks of exposure of TET (50 μg·L-1) caused histopathological lesion in the liver and intestine, further induced oxidative damage. Exposure of As(Ⅲ) (100 μg·L-1) caused local inflammation in the liver and induced oxidative stress in zebrafish. Continuous exposure of TET and As(Ⅲ) resulted in reduced condition factor and up-regulated oxidative damage, which may be related to the damage of the antioxidant defense system caused by TET. It's worth noting that recovery treatment after exposure of TET can reduce the toxicity caused by As(Ⅲ) on liver and intestine. The underlying mechanism may associate with the adaptation and cross-resistance of organisms to pollutants. The results of this study will provide a new perspective for evaluating the combined toxic effects of TET and As(Ⅲ).
摘要点击次数： 538 全文下载次数： 603
