李佳,张亮,刘杰,林甲,宋新新,江瀚,彭永臻,刘鑫淼,伊锋.城市污水处理厂缺氧池短程反硝化现象及影响因素研究[J].环境科学学报,2021,41(1):109-117
城市污水处理厂缺氧池短程反硝化现象及影响因素研究
- Investigation of the phenomenon and influencing factor of partial denitrification in anoxic zone of a municipal wastewater treatment plant
- 基金项目：国家水体污染控制与治理科技重大专项项目（No.2017ZX07102-003）
- 张亮
- 北京工业大学, 城镇污水深度处理与资源化利用国家工程实验室, 北京市污水脱氮除磷处理与过程控制工程技术研究中心, 北京 100124
- 彭永臻
- 北京工业大学, 城镇污水深度处理与资源化利用国家工程实验室, 北京市污水脱氮除磷处理与过程控制工程技术研究中心, 北京 100124
- 刘鑫淼
- 天津宁河首创污水处理有限公司, 天津 301500
- 摘要：调研了北方某城市污水处理厂缺氧池亚硝态氮积累的现象.该污水处理厂采用传统厌氧/缺氧/好氧（A/A/O）工艺，在缺氧池中存在稳定的短程反硝化过程，且缺氧池中亚硝态氮积累率最高可达88.4%.16S rRNA高通量测序分析表明Saccharibacteria_genera_incertae_sedis和Thauera可能是导致该厂缺氧池亚硝态氮积累的主要菌种，而短程反硝化现象出现的主要原因可能为外加碳源乙酸钠和系统较高的pH值.取活性污泥在COD/NO3--N为2~5条件下进行反硝化批次试验，结果表明硝态氮的还原速率均高于亚硝态氮的还原速率，且最大硝态氮到亚硝态氮的转换率均在50%左右.但碳源充足时，积累的NO2-会在NO3-被还原完后继续发生还原反应，从而导致最终亚硝态氮积累效果变差.本研究，以乙酸钠为碳源，COD/NO3--N为3可使反硝化过程获得最高亚硝态氮积累.因此，控制合适COD/NO3--N或缺氧反应时间是短程反硝化工艺运行的关键控制参数.本研究可为实际污水处理厂构建短程反硝化并进一步耦合厌氧氨氧化技术提供参考.
- Abstract：In this study, the phenomenon of nitrite accumulation in anoxic zone of a municipal wastewater treatment plant (WWTP) in northern china was investigated. The WWTP is a traditional anaerobic-anoxic-oxic (A/A/O) process, stable partial denitrification in the anoxic zone with the highest nitrite accumulation ratio of 88.4% was observed in anoxic zone. 16S rRNA amplicon sequencing analysis indicated that Saccharibacteria_genera_incertae_sedis and Thauera might be the main function bacterium for the nitrite accumulation in anoxic zone of this WWTP. And the addition of carbon source sodium acetate and the high pH value of the system might be the main reasons for the achievement of partial denitrification. Denitrification batch test was carried out with activate sludge under COD/NO3--N with 2~5, the result showed that the reduction rate of nitrate was always higher than that of nitrite, and the maximum nitrate to nitrite transformation ratio was all about 50%. However, when the carbon source was sufficient, the accumulated nitrite would continue to be reduced after the nitrate was completely reduced, leading to the final worse nitrite accumulation. In this research, with the sodium acetate as carbon source, the denitrification process obtained the highest accumulation of nitrite when COD/NO3--N was 3. Thus, controlling an appropriate COD/NO3--N or anoxic reaction time were the key control parameters for the operation of partial denitrification. This study can provide references for the construction of partial denitrification and further coupling of anammox process in real municipal WWTP.
