• 关键词：南方冬季  根际促生菌  镉  砷  巨菌草

• 基金项目：国家重点研发计划（No.2017YFD0801105）；农业部与财政部科研专项（农办财函〔2016〕6号）；湖南省重点学科建设项目（No.2006180）

• 李靖
• 1. 中南林业科技大学环境科学与工程学院, 长沙 410004;2. 中南林业科技大学稻米品质安全控制湖南省工程实验室, 长沙 410004

• 张平
• 1. 中南林业科技大学环境科学与工程学院, 长沙 410004;2. 中南林业科技大学稻米品质安全控制湖南省工程实验室, 长沙 410004

• 李倩
• 1. 中南林业科技大学环境科学与工程学院, 长沙 410004;2. 中南林业科技大学稻米品质安全控制湖南省工程实验室, 长沙 410004

• 廖柏寒
• 1. 中南林业科技大学环境科学与工程学院, 长沙 410004;2. 中南林业科技大学稻米品质安全控制湖南省工程实验室, 长沙 410004

• 彭佩钦
• 1. 中南林业科技大学环境科学与工程学院, 长沙 410004;2. 中南林业科技大学稻米品质安全控制湖南省工程实验室, 长沙 410004

• 李美芳
• 1. 中南林业科技大学环境科学与工程学院, 长沙 410004;2. 中南林业科技大学稻米品质安全控制湖南省工程实验室, 长沙 410004

• 梅金星
• 1. 中南林业科技大学环境科学与工程学院, 长沙 410004;2. 中南林业科技大学稻米品质安全控制湖南省工程实验室, 长沙 410004

• 摘要：优良菌种是微生物肥料的基础，研究微生物肥料中低温适应型菌株在低温条件下的促生性，对于提高南方冬闲时植物修复重金属污染农田具有重要意义.通过形态观察及18S rRNA基因序列分析，对菌株进行了鉴定，在此基础上进行植物盆栽试验，分析了3株不同根际促生菌单独施加及其与肥料（鸡粪+微量元素）配施对镉砷复合污染土壤中巨菌草迁移累积Cd、As的影响.分离菌株的初步鉴定结果表明，3株根际促生菌（M1、M2、M3）分别为云南木霉（Trichoderma yunnanense）、卵形孢球托霉（Gongronella butleri）、越橘间座壳菌（Diaporthe vaccinii）.纯培养试验表明，M1、M2、M3均可在南方冬季低温条件下生长.盆栽试验结果表明，与CK处理相比，单独施菌或肥料及菌株与肥料配施均能提高巨菌草茎叶生物量，分别提高14.12%~84.35%、13.36%、57.25%~143.13%.在所有处理中，M3与肥料配施后，植株茎叶生物量达到最大.不同处理可影响巨菌草对Cd和As的吸收累积，但对巨菌草各部位影响不同.单独施菌时，M3对巨菌草茎叶Cd、As积累量提升最多，分别提高145.83%、64.03%；当菌株与肥料配施时，M3与肥料配施对巨菌草茎叶Cd、As积累量提升最多，分别提高94.17%、142.45%.可见，3株根际促生菌在南方冬季均能提高巨菌草生物量，其中，菌株M3与肥料配施具有开发为有效治理镉砷复合污染土壤的微生物肥料的良好前景.

• Abstract：Excellent strains are the basis of microbial fertilizers. In order to improve the phytoremediation of soil heavy metal contaminated farmland, it is of great significance to study the growth-promoting ability of low-temperature-adapted strains in microbial fertilizers under low-temperature conditions during the winter slack in southern China. Strains were identified through morphological observation and 18S rRNA gene sequence analysis. On this basis, a pot experiment was carried out to study the effects of three different plant growth-promoting rhizobacteria (PGPR) applied alone or in combination with fertilizer(chicken manure + trace elements) on the migration and accumulation of cadmium (Cd) and arsenic (As) in pennisetum sinese in the soil contaminated with Cd and As. The preliminary identification results of the isolated strains showed that M1, M2, and M3 were Trichoderma yunnanense, Gongronella butleri, and Diaporthe vaccinii, respectively. The pure culture experiments showed that M1, M2, and M3 could grow under low temperature conditions in southern winter. The results of the pot experiment show that, compared with CK treatment, the biomass of the stems and leaves of pennisetum sinese treated with single application of strains or fertilizer, or combination application of strains and fertilizer increased by 14.12%~84.35%, 13.36%, 57.25%~143.13%, respectively. Among all treatments, the combined application of M3 and fertilizer resulted in the maximum biomass of stems and leaves of pennisetum sinese. Different treatments could affect the absorption and bioaccumulation of Cd and As in pennisetum sinese, but the effects on each part in various tissues of pennisetum sinese are different. When strains were applied alone, M3 resulted in the greatest accumulation of Cd and As in the stems and leaves in pennisetum sinese with increasing magnitudes of 145.83% and 64.03%, respectively. When strains are combined with fertilizers, the combined application of M3 and fertilizers showed the greatest accumulation of Cd and As in the stems and leaves in pennisetum sinese with increasing magnitudes of 94.17% and 142.45%, respectively. Overall, the results of this study indicated that three PGPR could effectively promoted the biomass of pennisetum sinese in southern winter. Among them, the strain M3 combined with fertilizer has good prospect of being developed as a microbial fertilizer for effective treatment of soil contaminated with Cd and As.

• Key words：southern winter  plant growth-promoting rhizobacteria(PGPR)  cadmium  arsenic  Pennisetum sinese

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