汤默然,李茹莹.异养硝化-好氧反硝化菌株的分离筛选及复配菌剂对河水的净化效果[J].环境科学学报,2021,41(7):2657-2663
异养硝化-好氧反硝化菌株的分离筛选及复配菌剂对河水的净化效果
- Isolation of heterotrophic nitrification-aerobic denitrification bacteria strains and purification of river water by mixed cultures
- 基金项目：国家水体污染控制与治理科技重大专项（No.2017ZX07106004）
- 汤默然
- 天津大学环境科学与工程学院, 天津 300350
- 李茹莹
- 天津大学环境科学与工程学院, 天津 300350
- 摘要：从天然河水中富集分离出8株异养硝化-好氧反硝化（HN-AD）菌株.将单菌株根据其自身的种属类别及脱氮性能复配成5种由不同菌株构成的菌剂，优选出脱氮效果最佳的复配菌剂-2，其包括6株菌株，分别为Pseudomonas stutzeri MR1，Pseudomonas sp. MR2，Pseudomonas sp. MR3，Pseudomonas balearica MR4，Klebsiella variicola MR6和Catellibacterium terrae MR8.将复配菌剂-2投加至CODCr/TN比分别为20和5的河水中，其对NO3--N的去除率分别为87.1%和97.5%，期间无NO2--N积累；对NH4+-N去除效果在第1 d分别达到96.6%和57.6%.复配菌剂可以获得对河水较高的反硝化脱氮效率，并可以强化河水中NH4+-N的去除，对实际河水的脱氮净化具有较强的应用潜能.
- Abstract：In this study, eight strains of heterotrophic nitrification-aerobic denitrification (HN-AD) strains were isolated from river water and then five mixed cultures were prepared according to the nitrogen removal performance and the classification of each strain. The mixed culture-2 performed better nitrogen removal capacity than others, which consisted of six strains of HN-AD bacteria including Pseudomonas stutzeri MR1, Pseudomonas sp. MR2, Pseudomonas sp. MR3, Pseudomonas balearica MR4, Klebsiella variicola MR6 and Catellibacterium terrae MR8. The mixed culture-2 was inoculated into the raw river water with CODCr/TN ratios of 20 and 5 respectively, achieving NO3--N removal efficiencies of 87.1% and 97.5%, respectively, without NO2--N accumulation. The NH4+-N removal efficiencies reached 96.6% and 57.6%, respectively, at the first day. The HN-AD mixed culture-2 could obtain high denitrification efficiency, and could enhance the NH4+-N removal, suggesting a potential for the nitrogen removal of practical river water.
