刘琴,信欣,周希,廖娟,段文瑶,解宗兰,叶恒.磁性纳米Fe3O4@C对SBR脱氮除磷性能及其微生物种群组成的影响[J].环境科学学报,2021,41(7):2664-2672
磁性纳米Fe3O4@C对SBR脱氮除磷性能及其微生物种群组成的影响
- Effects of Fe3O4@C nanoparticles on nitrogen and phosphorus removal performance and microbial community in a sequencing batch reactor
- 基金项目：四川省教育厅科研项目（No.17ZA0067）；大学生创新创业训练计划项目（No.202010621355）
- 刘琴
- 成都信息工程大学资源环境学院, 成都 610225
- 信欣
- 成都信息工程大学资源环境学院, 成都 610225
- 周希
- 成都信息工程大学资源环境学院, 成都 610225
- 廖娟
- 成都信息工程大学资源环境学院, 成都 610225
- 段文瑶
- 成都信息工程大学资源环境学院, 成都 610225
- 解宗兰
- 成都信息工程大学资源环境学院, 成都 610225
- 叶恒
- 成都信息工程大学资源环境学院, 成都 610225
- 摘要：为探究磁性纳米Fe3O4@C对序批式活性污泥反应器（SBR）污水处理系统脱氮除磷性能的影响，建立了2个相同的SBR（编号分别为0号和1号），其中，0号反应器未投加任何磁性材料（对照组），1号反应器中投加0.5 g·L-1的Fe3O4@C（实验组），并采用高通量测序和实时定量PCR（qPCR）技术对2个反应器内生物种群结构及关键脱氮除磷功能菌群进行分析.结果表明：①Fe3O4@C对SBR除污性能有显著影响，其中，1号反应器化学需氧量（COD）的去除性能得到增强，24 d后去除率稳定在90%左右；0号和1号反应器总氮（TN）平均去除率分别为35.83%和52.18%；从第43 d起，1号反应器除磷性能明显高于0号反应器，运行70 d后，0号和1号反应器对总磷（TP）的平均去除率分别为47.52%和56.33%.②添加Fe3O4@C材料后，反应器内变形菌门（Proteobacteria）的相对丰度从23.91%增加至32.14%，优势脱氮菌门Planctomycetes、Nitrospirae的相对丰度分别从2.66%、0.46%增加至5.16%、2.23%.③添加Fe3O4@C后，SBR内细菌总数明显增多，16S基因拷贝数从1.77×107 copies·g-1增长到4.21×107 copies·g-1；各功能菌数量也有大幅度增长，除磷功能基因PAO增加了3.4倍，脱氮功能基因nirS、Nitrospira、Nitrobacter、AOB分别增长了2.3、2.4、4.7和572倍.研究表明，磁性纳米Fe3O4@C能有效促进SBR脱氮除磷性能，可为SBR工艺的优化改进提供理论支撑.
- Abstract：The identical two sequencing batch reactors (SBRs) (named by No.0 and No.1) were set up in order to study the effects of magnetic nano-Fe3O4@C on the performances of biological nitrogen and phosphorus removal in a sequencing batch reactor (SBR). The dosage of 0.5 g·L-1 nano-Fe3O4@C were added to reactor-1, and no addition of magnetic material in reactor-0. High throughput sequencing and real-time quantitative PCR (qPCR) were used to analyze the key microbial populations in the two reactors. The results showed that: ①Fe3O4@C had significant influence on the performance of SBR. Among them, COD removal in reactor-1 was enhanced, and the efficiency was stable at about 90% after 24 days; the average efficiency of TN in reactor-0 and -1 were 35.83% and 52.18%, respectively. From day 43, the phosphorus removal performance of reactor 1 was significantly higher than reactor 0. From day 70, the average efficiency of TP in reactor-0 and -1 were 47.52% and 56.33%, respectively. ② After adding Fe3O4@C, the relative abundance of Proteobacteria increased from 23.91% to 32.14%; the dominant nitrogen removal bacteria Planctomycetes and Nitrospirae increased from 2.66%, to 5.16% and from 0.46% to 2.23%, respectively. ③ After adding Fe3O4@C, the total number of bacteria in SBR increased significantly, and the number of 16s gene copies increased from 1.77×107 to 4.21×107 copies·g-1. the number of functional bacteria also increased significantly, and the phosphorus removal gene PAO increased 3.4-fold; the genes of nirS, Nitrospira, Nitrobacter, and AOB increased by 2.3, 2.4, 4.7, and 572 times, respectively. Experimental results showed that magnetic nano-Fe3O4@C can effectively promote the nitrogen and phosphorus removal performance in SBRs, providing theoretical support for the optimization and improvement of SBR process.
