李培瑞,汪素芳,赵晓婵,周爱娟,孔鑫,段润斌,岳秀萍.零价铁预厌氧协同MFC强化降解吲哚性能与功能菌群分析[J].环境科学学报,2021,41(7):2673-2681
零价铁预厌氧协同MFC强化降解吲哚性能与功能菌群分析
- The performance and functional microbial community analysis in a combination system of zero-valent iron pre-anaerobic treatment and MFC for strengthening the degradation of indole
- 基金项目：国家自然科学基金项目（No.21707099，51908396）；山西省高等学校创新计划项目（No.2019L0161）
- 李培瑞
- 太原理工大学环境科学与工程学院, 太原 030024
- 汪素芳
- 太原理工大学环境科学与工程学院, 太原 030024
- 赵晓婵
- 太原理工大学环境科学与工程学院, 太原 030024
- 周爱娟
- 太原理工大学环境科学与工程学院, 太原 030024
- 孔鑫
- 太原理工大学环境科学与工程学院, 太原 030024
- 段润斌
- 太原理工大学环境科学与工程学院, 太原 030024
- 岳秀萍
- 太原理工大学环境科学与工程学院, 太原 030024
- 摘要：通过零价铁（ZVI）预厌氧与微生物燃料电池（MFC）的协同作用，研究其对单一基质碳源吲哚的降解特性、MFC产电性能及群落分析.结果表明：ZVI（4 g·L-1）的预厌氧可明显促进吲哚（250 mg·L-1）在MFC体系中的降解；其中吲哚与TOC在96 h内的降解率分别为97.17%和89.50%，且吲哚的降解符合一级反应动力学模型；体系中MFC最大输出电压和功率密度可达600 mV和439.55 mW·m-2；通过LC-MS分析，吲哚在协同体系中的主要中间代谢产物为靛红和水杨酸.高通量测序结果表明，ZVI预厌氧体系的引入有利于MFC体系中梭菌（Clostridium sensu stricto）、链霉菌（Streptomyces sp.）、热单胞菌（Thermomonas）的富集与吲哚的厌氧降解；同时促进假单胞菌（Pseudomonas）和梭菌等在代谢过程中的电子转移，提高了MFC的产电性能.
- Abstract：The objectives of this study were to investigate the indole degradation characteristics, the electricity generation performance of microbial fuel cell (MFC), and microbial community by combinating zero-valent iron (ZVI) anaerobic treatment and MFC. The results showed that the pre-anaerobic treatment with the ZVI of 4 g·L-1 remarkably promoted the degradation of indole with the initial concentration of 250 mg·L-1 in the MFC system. The degradation of indole and total organic carbon was 97.17% and 89.50% in 96 hours, respectively. The first-order reaction kinetic model well described the indole degradation. The maximum output voltage and power density of the MFC in the synergetic system were found to be 600 mV and 439.55 mW·m-2, respectively. LC-MS analysis results indicated that isatin and salicylic acid were the primary intermediate metabolites during indole degradation in the synergetic system. The results of high-throughput sequencing revealed that the ZVI pretreatment obviously boosted the growth of Clostridium sensu stricto, Streptomyces sp., and Thermomonas in the MFC system and the degradation of indole. Additionally, the ZVI pretreatment promoted the electron transfer in the metabolic processes of Pseudomonas and Clostridium sensu stricto resulting in the higher electricity generation performance of MFC.
