杨婉静,潘杨,陈越,张星宇,倪敏.胞外聚合物在生物膜同步去除/富集磷酸盐系统中的作用[J].环境科学学报,2021,41(9):3437-3445
胞外聚合物在生物膜同步去除/富集磷酸盐系统中的作用
- The role of extracellular polymer substances in simultaneous removal/enrichment of phosphate in biofilms system
- 基金项目：国家自然科学基金项目（No.51778390，51938010）；江苏水处理技术与材料协同创新中心预研项目（No.XTCXSZ2020-4）；江苏省研究生科研创新计划（No.KYCX19_2032）
- 杨婉静
- 苏州科技大学环境科学与工程学院, 苏州 215009
- 潘杨
- 1. 苏州科技大学环境科学与工程学院, 苏州 215009;2. 城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
- 陈越
- 苏州科技大学环境科学与工程学院, 苏州 215009
- 张星宇
- 苏州科技大学环境科学与工程学院, 苏州 215009
- 倪敏
- 苏州科技大学环境科学与工程学院, 苏州 215009
- 摘要：以基于同步去除/富集磷酸盐的厌氧/好氧交替生物膜序批式反应器内生物膜为研究对象，研究了胞外聚合物（EPS）内磷含量形态及生物膜内微生物种群变化，探究EPS在生物膜去除/富集磷酸盐中的作用及其与微生物种群之间的联系.结果表明，生物膜反应器在厌氧外加COD为200 mg·L-1的条件下富集到了磷浓度为120.95 mg·L-1的富集液.EPS在生物膜吸/释磷过程中发挥重要作用，EPS磷含量占生物膜磷含量的69.16%~79.00%，31P核磁共振实验表明ortho-P为EPS内主要磷形态，占比为85.47%~88.60%.高通量测序结果表明生物膜内微生物种群变化明显，Candidatus_Competibacter为优势菌属，其丰度由1.23%增至38.87%，有利于形成更具粘性的EPS进而黏附在生物膜上，可能促进EPS在吸/释磷中发挥作用；暖绳菌科为优势聚磷菌，随实验温度升高，其丰度由5.29%减至4.90%.
- Abstract：An anaerobic/aerobic alternate biofilm sequence-batch reactor was investigated for simultaneous removal/enrichment of phosphate.The role of extracellular polymer substances (EPS) in phosphate removal/enrichment in biofilm and its relationship with microbial populations were explored through studying the content and morphology of phosphorus in EPS and the changes of microbial population in biofilm. The results showed that the phosphate concentration in the bioflum enrichment was up to 120.95 mg·L-1 in the biofilm reactor under 200 mg·L-1 COD in anaerobic phase. EPS played an important role in the phosphorus absorption/release process of biofilm, and the phosphorus content in EPS accounted for 69.16%~79.00% of the phosphorus content in biofilm. 31P nuclear magnetic resonance experiments results showed that Ortho-P was the main form of phosphorus in EPS, accounting for 85.47%~88.60%. High-throughput sequencing results showed that the microbial population in the biofilm changed significantly. Candidatus_Competibacter was the dominant genus, and its abundance increased from 1.23% to 38.87%, which was conducive to the formation of more viscous EPS and its adhesion to the biofilm, and may enhance the role of EPS in phosphorus absorption and release. Caldilineaceae was the dominant phosphorus accumulating organisms and its abundance decreased from 5.29% to 4.90% with the increase of temperature.
