• 关键词：厌氧氨氧化  富集培养  移动床生物膜反应器(SMBBR)  部分反硝化

• 基金项目：陕西省重点研发计划（No.2019ZDLSF06-05）

• 姚雪薇
• 西安建筑科技大学环境与市政工程学院, 西安 710055

• 邵贤明
• 西安建筑科技大学环境与市政工程学院, 西安 710055

• 王康舟
• 西安建筑科技大学环境与市政工程学院, 西安 710055

• 吕恺
• 西安建筑科技大学环境与市政工程学院, 西安 710055

• 彭党聪
• 1. 西安建筑科技大学环境与市政工程学院, 西安 710055;2. 西北水资源与环境生态教育部重点实验室, 西安 710055

• 摘要：通过接种某城市污水处理厂好氧池生物膜，采用NH₄⁺-N+NO₂^--N （SMBBR-1）和NH₄⁺-N+NO₃^--N+HAc （SMBBR-2）两种进水基质启动厌氧氨氧化序批式移动床生物膜反应器（Sequencing Moving Bed Biofilm Reactor，SMBBR），研究不同基质条件下反应器的启动特性.结果表明，两反应器在运行100 d后均成功启动并稳定运行，在进水负荷分别为0.83和0.32 kg·m^-3·d^-1（以N计）的条件下，氮去除率分别达到81.82%±1.20%和66.35%±4.79%.活性测定结果显示，SMBBR-1和SMBBR-2中Anammox活性分别达到6448.32和1980.32 mg·m^-2·d^-1，表明Anammox菌被成功富集.高通量结果显示，SMBBR-1和SMBBR-2中启动成功后的Anammox菌由Ca.Brocadia和Ca.Jettenia组成，其中，Ca.Brocadia占比分别为11.02%和7.57%，Ca.Jettenia占比分别为2.07%和0.56%.除Anammox菌外，SMBBR-2中还包括Thauera（2.84%）和Flavobacterium菌（0.66%），其为部分反硝化菌的主导菌属.本研究表明，虽然两种不同基质的启动办法各有利弊，但其均能实现厌氧氨氧化SMBBR的启动，可为主流系统内的Anammox菌快速富集培养提供技术支撑.

• Abstract：To investigate the characteristics of anammox enrichment with different substrates, two SMBBRs with aerobic biofilm taken from municipal wastewater treatment were established. One was fed with ammonium and nitrite (SMBBR-1), the other was fed with ammonium,nitrate and acetic acid (SMBBR-2). The long-term operation results showed 81.82%±1.20% and 66.35%±4.79% of nitrogen removal efficiencies were stably obtained under the nitrogen loading rate (NLR) of 0.83 and 0.32 kg·m^-3·d^-1 for SMBBR-1 and SMBBR-2 after 100 days' operation. The anammox activities of SMBBR-1 and SMBBR-2 could reach 6448.32 and 1980.32 mg·m^-2·d^-1, respectively, which suggested that anammox bacteria were successfully enriched in two SMBBRs. The high-throughput sequencing results revealed Ca. Brocadia and Ca. Jettenia were the predominance for anammox bacteria in SMBBR-1 and SMBBR-2. The relative abundance of Ca. Brocadia were 11.02% and 7.57%, and the abundance of Ca. Jettenia were 2.07% and 0.56%, respectively. The Thauera and Flavobacterium were also detected with relative abundance of 2.84% and 0.66% in SMBBR-2, which were the dominated species of partial denitrifying bacteria. This study showed that enrichment methods with different substrates for anammox SMBBR start-up had the advantages and disadvantages. However, these two substrates strategies could be used for reactor establishment and provided support for anammox enrichment in mainstream.

• Key words：anaerobic ammonium oxidation (anammox)  enrichment  sequencing moving bed biofilm reactor (SMBBR)  partial denitrification

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