• 关键词：硫化氢  酿酒酵母  亚砷酸钠  硫氢化钠

• 基金项目：国家自然科学基金（No.21307087）；山西省应用基础研究计划项目（No.201601D021104）；山西省高校科技创新项目（No.2019L0820）；太原师范学院大学生创新项目（No.CXCY2160）

• 李海燕
• 太原师范学院生物系, 晋中 030619

• 吴丽华
• 太原师范学院生物系, 晋中 030619

• 摘要：以模式生物酿酒酵母为研究对象，采用醋酸铅试纸条法、BIGGY琼脂及qRT-PCR技术检测了不同浓度亚砷酸钠胁迫下H₂S产量及产H₂S酶编码基因表达的变化，以探讨砷化物对酵母细胞H₂S代谢的影响.结果显示，0.5~2 mmol·L^-1的亚砷酸钠可诱导酵母细胞内源H₂S的产生，且H₂S合成相关基因的相对表达量与对照相比均有不同程度的上调.经0.05~0.2 mmol·L^-1的外源H₂S预处理后，酵母细胞对砷的耐受性明显提高.研究表明，亚砷酸钠可影响酵母细胞H₂S代谢，而H₂S含量可在一定程度上调控砷对酵母的毒性.

• Abstract：The model organism, Saccharomyces cerevisiae, was used to investigate the effects of arsenite on H₂S metabolism in yeast cells. Changes in H₂S production, and the expression of genes encoding H₂S-producing enzymes under the stress of different concentrations of sodium atsenite were detected using the lead acetate test strip method, bismuth sulfite glucose glycine yeast (BIGGY) agar, and qRT-PCR. The experiment results showed that sodium arsenite at 0.5~2 mmol·L^-1 induced endogenous H₂S production in yeast cells, and the relative expression levels of H₂S synthesis-related genes were all upregulated to different levels compared to the control. The tolerance of yeast cells to arsenic was significantly increased after pretreatment with 0.05~0.2 mmol·L^-1 of exogenous H₂S. The results suggest that sodium arsenite can affect yeast cell H₂S metabolism, and H₂S can regulate the toxicity of arsenic to yeast to some extent.

• Key words：hydrogen sulfide (H₂S)  Saccharomyces cerevisiae  sodium arsenite  sodium hydrosulfide (NaHS)

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