陈玲,贾敏,何若男,余静,吴兵.砷和二氯乙酰胺联合暴露下斑马鱼脏器富集及毒性的性别二态性研究[J].环境科学学报,2021,41(8):3384-3393
砷和二氯乙酰胺联合暴露下斑马鱼脏器富集及毒性的性别二态性研究
- The sex dimorphism in accumulation and combined toxicity of arsenic and dichloroacetamide in adult zebrafish
- 基金项目：国家重点研发计划（2017YFE0107200）；国家自然科学基金（No.21976087，22022604）
- 陈玲
- 南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
- 贾敏
- 南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
- 何若男
- 南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
- 余静
- 南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
- 吴兵
- 南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
- 摘要：砷（As）是全球性毒害污染物，可与消毒副产物共存于水环境中，但目前缺乏两者对水生生物的系统联合毒性效应评估数据.本研究选用毒性较强的含氮消毒副产物—二氯乙酰胺（DCAcAm）为代表，研究其与无机三价As在不同性别成年斑马鱼肝脏、肠道、脑和鳃等器官中的联合毒性效应.结果表明，100 μg·L-1 As和300 μg·L-1 DCAcAm单一和联合暴露主要累积于肝脏，并在肝脏诱导最强的氧化损伤及炎症反应.As单一暴露对雌性斑马鱼表现出更强毒性，而DCAcAm单一暴露则在雄性斑马鱼中诱导更强毒性，两者的联合毒性效应在两种性别斑马鱼肝脏中均减弱.在雄性斑马鱼肠道、脑和鳃中，As和DCAcAm的毒性效应相互独立；而在雌性斑马鱼脑和鳃中联合毒性减弱，肠道中联合毒性增强.本研究结果可为As和DCAcAm联合毒性评估及健康风险评价提供数据支撑，对DCAcAm暴露的性别二态性响应的首次表征，可为DBPs类污染毒性效应评估提供另一维度的参考数据.
- Abstract：Arsenic (As) is a global toxic pollutant and can coexist with disinfection by-products (DBPs) in the environment. However, there is a lack of data about their combined toxicity. In this study, the dichloroacetamide (DCAcAm), a highly toxic representative nitrogen DBPs, was selected to study the combined toxicity with inorganic trivalent As on liver, gut, brain, and gill in male and female adult zebrafish. The results showed that 100 μg·L-1 As or/and 300 μg·L-1 DCAcAm exposure mainly accumulated in the liver, and induced the strongest oxidative damage and inflammation in the liver. The combined toxicity of As and DCAcAm were weakened in the livers of male and female zebrafish. In the gut, brain and gills of male zebrafish, the toxicity of As and DCAcAm was independent of each other, while the combined toxicity of the two was weakened in the brain and gills of female zebrafish and enhanced in gut of female zebrafish. The results of this study can provide data support for the combined toxicity assessment and health risk assessment of As and DCAcAm, and the characterization of sex dimorphic response to DCAcAm exposure for the first time can provide another dimension of data for the evaluation of the DBPs toxicity.
