研究报告

  • 赵庆庆,解金昆,高永超,张闻,王加宁,陈贯虹.不同水文条件下黄河口滨海湿地土壤真菌群落的分布特征[J].环境科学学报,2022,42(1):95-103

  • 不同水文条件下黄河口滨海湿地土壤真菌群落的分布特征
  • The distribution pattern of soil fungal community in coastal wetlands with different hydrologic conditions in the Yellow River Estuary
  • 基金项目:国家自然科学基金青年科学基金(No.41807396);山东省自然科学基金(No.ZR2019BD007);山东省科学院青年基金(No.2020QN0015);国家重点研发计划(No.2019YFC1804103)
  • 作者
  • 单位
  • 赵庆庆
  • 齐鲁工业大学(山东省科学院),山东省科学院生态研究所,山东省应用微生物重点实验室,济南 250103
  • 解金昆
  • 齐鲁工业大学(山东省科学院),山东省科学院生态研究所,山东省应用微生物重点实验室,济南 250103
  • 高永超
  • 齐鲁工业大学(山东省科学院),山东省科学院生态研究所,山东省应用微生物重点实验室,济南 250103
  • 张闻
  • 齐鲁工业大学(山东省科学院),山东省科学院生态研究所,山东省应用微生物重点实验室,济南 250103
  • 王加宁
  • 齐鲁工业大学(山东省科学院),山东省科学院生态研究所,山东省应用微生物重点实验室,济南 250103
  • 陈贯虹
  • 齐鲁工业大学(山东省科学院),山东省科学院生态研究所,山东省应用微生物重点实验室,济南 250103
  • 摘要:为了探究不同水文条件下黄河口滨海湿地土壤真菌群落多样性和结构特征,通过内转录区间(ITS)高通量测序技术对淡水恢复芦苇湿地(FPW)、非淹水芦苇湿地(NPW)、潮汐芦苇湿地(TPW)和盐地碱蓬湿地(TSW)土壤的真菌群落多样性和结构进行对比分析.结果表明,非淹水芦苇湿地土壤中真菌OTUs数量最高(902),但4种湿地土壤检出的真菌物种数之间无显著性差异(p>0.05).Shannon指数和Simpson(1-D)指数表明,潮汐芦苇湿地和盐地碱蓬湿地土壤真菌群落多样性显著高于淡水恢复芦苇湿地和非淹水芦苇湿地(p<0.05).4种湿地0~10 cm土壤中优势度最高的真菌门为子囊菌门(20.51%~75.69%),而子囊菌纲则在0~5 cm土壤真菌群落中占比最高(15.37% ~52.38%).线性判别分析(LEfSe)表明,盐地碱蓬湿地土壤中具有特异性丰度值的真菌物种数多于其他3种湿地.曼特尔分析和斯伯曼相关分析证实土壤理化性质是影响真菌群落结构的重要因素.受潮汐影响的芦苇和碱蓬湿地相比淡水恢复芦苇湿地和非淹水芦苇湿地具有更高的真菌群落多样性,该研究结果可以为湿地修复策略的选择提供理论指导.
  • Abstract:Sequences of Internal Transcribed Spacer 1(ITS1)in soils from four different wetlands were determined to clarify the response of fugal communities to different hydrologic conditions. Soil samples from 0~5 cm and 5~10 cm depths were collected from flooded freshwater-restored Phragmites australis wetlands (FPW), nonflooding Phragmites australis wetlands (NPW), tidal Phragmites australis wetlands (TPW) and tidal Suaeda salsa wetlands (TSW), respectively. The results showed that there were no significant differences in the number of fungal OTUs among the four wetlands (p>0.05), but the highest value of OTUs number was found in NPW(902). The Shannon index and Simpson(1-D) index indicated that the diversity of fungal community in soils from TPW and TSW were significantly higher than that of soils from FPW and NPW (p<0.05). Ascomycota and Sordariomycetes were the predominant fungal phylum in soils of 0~10 cm depth and the most dominant fugal class in soils of 0~5 cm depth, respectively. The Linear Discriminant Analysis (LefSe) suggested that the number of discriminant fugal species were higher in soils from TSW than those from other three wetlands. The Mantel test and Spearman correlation analysis confirmed that soil properties are important factors influencing the structure of fungal community. Higher diversity of fungal community was observed in soils from two tidally affected wetlands than freshwater wetlands and non-flooding wetlands, which potentially provides guidelines for the choice of wetland restoration strategy.

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