• 关键词：生物电化学系统  胞外电子传递  细胞色素c  石墨烯/血红素仿生涂层  阳极修饰

• 基金项目：国家自然科学基金面上项目（No. 42077218）

• 詹骥
• 福建农林大学资源与环境学院，福州 350002

• 靖宪月
• 福建农林大学资源与环境学院，福州 350002

• 刘星
• 福建农林大学资源与环境学院，福州 350002

• 周顺桂
• 福建农林大学资源与环境学院，福州 350002

• 摘要：阳极输出效率是决定生物电化学系统（BESs）能否实现工程化应用的基础和关键.但微生物/阳极界面的低效电子传递阻碍了BESs整体输出效率的进一步提升.本研究通过模拟细胞色素c构建了一种石墨烯/血红素（GN/Hemin）复合仿生材料修饰的高性能BESs阳极.通过监测电活性菌Shewanella loihica PV-4的电能输出动态，证实了修饰涂层对微生物/阳极界面电子传递的促进功效.结果表明，菌株PV-4利用GN/Hemin修饰阳极的最大电流密度为7.28 μA·cm^-2，比无修饰阳极电流（0.65 μA·cm^-2）高出10.2倍.电化学分析显示，GN/Hemin涂层显著降低了微生物/阳极界面的电荷转移阻抗，并显著提高了阳极的电化学催化活性.此外，GN/Hemin涂层能够使无产电性能的Geobacter sulfurreducens DL1突变株（Gs-ΔomcZ）恢复产电，表明GN/Hemin具有类OmcZ功能.本研究为BESs提供了一种简易的高性能阳极仿生修饰策略，同时考虑到OmcZ独特的高导电性能，石墨烯/血红素涂层的类OmcZ功能的发现将具有重要的科学和应用价值.

• Abstract：The anode output efficiency is the fundament and key to determine whether bioelectrochemical systems （BESs） can be used for engineering application. However， the inefficient electron transfer at the microbe/anode interface hinders the further improvement of the overall output efficiency of BESs. In this study， a biomimetic graphene/hemin （GN/Hemin） composite was synthesized to simulate cytochrome c， thereafter was used to modify the anode of BESs. By monitoring the current generation of the electroactive bacterium Shewanella loihica PV-4， the effect of the biomimetic coating on promoting electron transfer at the microbe/anode interface was confirmed. The results showed that the maximum current density of the GN/Hemin modified anode was 7.28 μA·cm^-2， which was 10.2 times higher than that of unmodified anode （0.65 μA·cm^-2）. Electrochemical analysis showed that the GN/Hemin coating significantly reduced the charge transfer resistance of the microbe/anode interface but improved the electrochemical catalytic activity of the anode. Furthermore， the GN/Hemin coating restored the current generation of the Geobacter sulfurreducens current generation deficient mutant strain （Gs-ΔomcZ）， indicating that GN/Hemin has an OmcZ-like function. This study provides a facile anode modification strategy using biomimetic coating to construct high-performance BESs. Meanwhile， considering the unique high conductivity of OmcZ， the discovery of the OmcZ-like function of graphene/heme coating has important scientific and application value.

• Key words：bioelectrochemical systems  extracellular electron transfer  cytochrome c  graphene/heme biomimetic coating  anode modification

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