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研究报告

张疆雨,李明翰,李泽昌,周春艳,罗芳,潘一帆,刘薇.3种新烟碱类杀虫剂对人间充质干细胞基因表达谱的影响[J].环境科学学报,2022,42(2):450-457

Effects of three neonicotinoids on gene expression profile of human mesenchymal stem cells

摘要：啮齿类动物毒理学的初步研究结果提示新烟碱类化学品暴露可能对人体健康造成危害，但健康效应、毒性机制和分子毒理学靶标尚不清楚.选用人骨髓间充质干细胞体外模型，将其暴露于吡虫啉（IMI）、烯啶虫胺（NIT）和啶虫脒（ACE）3种典型新烟碱类杀虫剂，利用基因加权共表达网络分析细胞基因表达谱的变化.基因加权共表达网络分析将基因分为7个模块，其中5个模块基因表达模式与对照组有显著差异（p<0.05）.从上述5个模块中共富集出57条通路与236个生物学过程，主要涉及脂肪酸代谢、葡萄糖代谢、凋亡及神经系统.从各模块中筛选出连通性最高的20个关键基因，其中，Blue及Red模块中关键基因主要包括神经生长因子（NGF）、丝氨酸蛋白酶抑制剂家族B成员2（SERPINB2）及酰基辅酶A脱氢酶中链（ACADM）.本研究提示了新烟碱类杀虫剂对人体健康的潜在敏感靶标，可为该类化学品的健康风险评价提供科学依据.

Abstract：The results of rodent toxicology suggested that exposure to neonicotinoids （NEOs） may cause harm to human health， but the health effects， toxicity mechanism and molecular toxicological targets were still unclear. In the present study， human bone mesenchymal stem cells （hBMSCs） were employed as an in vitro model. The gene profiles in hBMSCs disturbed by imidacloprid （IMI）， nitenpyram （NIT） and acetamiprid （ACE） were analyzed by weighted gene co-expression network analysis （WGCNA）. The genes were divided into seven modules by WGCNA and the gene expression patterns of red， green， yellow， blue and brown modules were significantly different from those of the control group（p<0.05）. A total of 57 pathways and 236 biological processes were enriched from the above mentioned five modules， mainly involving fatty acid metabolism， glucose metabolism， apoptosis and nervous system. Twenty key genes with the highest connectivity were identified from each module. The key genes in blue and red modules mainly include NGF， SERPINB2 and ACADM. The present study illustrated the potential sensitive targets of NEOs to human health， providing scientific basis for the toxicological study and health risk assessment of NEOs.

Key words：neonicotinoids（NEOs） human bone mesenchymal stem cells（hBMSCs） fatty acid metabolism nervous system weighted gene co-expression network analysis（WGCNA）