• 关键词：抗生素抗性基因（ARGs）  汾河水体  赋存特征  环境因子

• 基金项目：山西省基础研究计划，自然科学研究面上项目（No.202103021224031）；国家自然科学基金（No.41771548）；国家联合基金重点项目（No.U1910207）

• 黄高琪
• 山西大学黄土高原研究所，太原 030006

• 石伟
• 山西大学黄土高原研究所，太原 030006

• 李君剑
• 山西大学黄土高原研究所，太原 030006

• 摘要：抗生素抗性基因（Antibiotic resistance genes，ARGs）被认为是一种新型污染物，而水环境系统是ARGs释放和传播的重要媒介.为探究 汾河水体环境中ARGs的赋存特征及分布特征，选取汾河水体从源头到入黄口的12个位点，对环境因子、ARGs的丰度及多样性，以及二者之间的关系进行研究.结果表明，汾河水体中检测到62种ARGs、6种可移动遗传元件（Mobile genetic elements， MGEs）；ARGs和MGEs相对丰度在水体中呈现中游>下游>上游的趋势.bla_TEM和intⅠ1分别是ARGs和MGEs中占主导的基因.bla_TEM与tetW、tetB、tetM、ΣARGs、ΣMGEs均呈 正相关；整合子intⅠ1与bla_TEM、sulⅠ、sulⅡ、ΣARGs之间均呈正相关（p<0.05），说明整合子对汾河水体ARGs的转移和富集具有重要作用.pH与sulⅠ、sulⅡ及ΣMGEs呈负相关（p＜0.05）；COD和TN与bla_TEM、tetW、floR、marR及ΣARGs呈正相关（p<0.05），说明汾河污染可能对ARGs 富集及在细菌群落之间的水平转移有影响，但同时抗性基因的赋存及分布状况也可能受到其他环境因子的影响.

• Abstract：Antibiotic resistance genes （ARGs） has been considered to be a new contaminant， and the water environment system is an important medium for the release and spread of ARGs. In order to explore the occurrence and distribution of ARGs in the water environment of the Fenhe River， 12 sample sites from the source to the entrance to the Yellow River were selected to study the environmental factors， the abundance and the diversity of ARGs， and their relationship. The results showed that 62 kinds of ARGs and 6 kinds of mobile genetic elements （MGEs） were detected in the water environment of the Fenhe River. The relative abundances of ARGs and MGEs followed the trend of midstream>downstream>upstream. bla_TEM and intⅠ1 were the dominant genes in ARGs and MGEs， respectively. bla_TEM positively correlated with tetW， tetB， tetM， ΣARGs and ΣMGEs （p<0.05）. The integron intI1 positively correlated with bla_TEM， sulⅠ， sulⅡ， and ΣARGs （p<0.05）， indicating that integron plays an important role in the transfer and enrichment of ARGs in the Fenhe River. Environmental factor pH negatively correlated with sulⅠ， sulⅡ and ΣMGEs （p>0.05）. COD and TN positively correlated with blaTEM， tetW， floR， marR and ΣARGs （p<0.05）， which indicates that the pollution may affect ARGs enrichment and the horizontal transfer among bacterial communities in the Fenhe River. But meanwhile the occurrence and distribution of ARGs may also be affected by other environmental factors.

• Key words：antibiotic resistance genes（ARGs）  Fenhe River  occurrence characteristics  environmental factors

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