• 关键词：剩余污泥  木耳菌糠  预处理  厌氧发酵  水解酸化

• 基金项目：山西省自然科学基金（No.201901D111216）；山西省重点研发计划重点项目（No.201903D211012-05）；食用菌山西省科技创新重点团队（No.201805D131009）；山西省优秀博士来晋工作奖励基金（No.SXYBKY201803）；山西农业大学科技创新基金项目（No.2018YJ39）；山西省1331工程资助（No.20211331-15）；山西省研究生教育创新项目（No.2020SY189）

• 何小芳
• 山西农业大学，资源环境学院，太谷 030800

• 张海波
• 山西农业大学，资源环境学院，太谷 030800

• 程红艳
• 山西农业大学，资源环境学院，太谷 030800

• 任元森
• 山西农业大学，资源环境学院，太谷 030800

• 田鑫
• 山西农业大学，资源环境学院，太谷 030800

• 罗渊
• 山西农业大学，资源环境学院，太谷 030800

• 刘娜
• 山西农业大学，资源环境学院，太谷 030800

• 王雨萌
• 山西农业大学，资源环境学院，太谷 030800

• 摘要：∶为克服单一剩余活性污泥（WAS）发酵产酸效率低的问题和资源化利用废弃木耳菌糠（SMS），本文对热-碱预处理污泥（PWS）和菌糠进行共发酵，研究预处理和菌糠添加对共发酵体系的产酸性能影响.试验基于原料的总固体含量（TS）共设置6个处理，分别为污泥（WAS∶SMS=1∶0）、预处理污泥（PWS∶SMS=1∶0）、菌糠（PWS∶SMS=0∶1）、预处理污泥∶菌糠=1∶1（PWS∶SMS=1∶1）、污泥∶菌糠=1∶2（WAS∶SMS=1∶2）以及预处理污泥∶菌糠=1∶2（PWS∶SMS=1∶2），测定并分析了挥发性脂肪酸（VFA）、氨态氮、总磷、SCOD、酶等指标变化.结果表明∶PWS∶SMS=1∶2时，VFAs产量最高，可达5496.70 mg·L^-1，分别较单独污泥和单独菌糠产酸量提升了56.71%、54.97%；同时，该处理的氨态氮、正磷酸盐、SCOD的含量、α-葡萄糖苷酶和蛋白酶的相对活性明显高于其它处理组.预处理和菌糠的添加均能提高共发酵产酸性能，预处理可破坏污泥和细胞结构，促使SCOD、氨态氮和正磷酸盐释放，为水解酶提供了充足的反应底物；菌糠的添加则可为水解微生物提供合适的C/N，促进水解微生物大量繁殖，有利于分泌水解酶.热碱预处理污泥与菌糠构成的共发酵体系可高效转化污泥与菌糠固体废弃物为发酵代谢产物VFAs，为二者资源化利用提供一定的理论参考.

• Abstract：In order to solve the problem of low acid production efficiency， the anaerobic co-fermentation of thermo-alkali pretreated waste sludge （PWS） and SMS was carried out. Then the effects of thermo-alkali pretreatment and the addition of SMS on the acid production performance of the co-fermentation system were investigated. A total of 6 treatments were set up， namely WAS （WAS∶SMS=1∶0 and PWS∶SMS=1∶0）， SMS （PWS∶SMS=0∶1） and the co-fermentation of PWS and SMS （PWS∶SMS=1∶1； WAS∶SMS=1∶2 and PWS∶SMS=1∶2）. The changes of volatile fatty acids （VFA）， ammonia nitrogen， phosphorus， SCOD and key enzymes were measured and analyzed. The results showed that the highest yield of VFAs， up to 5496.70 mg?L^-1， was achieved under PWS∶SMS at 12， which was 56.71% and 54.97% respectively higher than that of WAS and SMS alone. At the same time， the contents of ammonia nitrogen， orthophosphate， SCOD and the relative activities of α -glucosidase and protease in this treatment were significantly higher than those in other treatments. Thus， both the thermo-alkali pretreatment and the addition of SMS can improve the acid production of co-fermentation system. The possible reason is that the pretreatment can destroy cell structure of WAS， promote the release of SCOD， ammonia nitrogen and orthophosphate， and provide sufficient reaction substrate for the hydrolysis. The addition of SMS can provide suitable C/N for hydrolytic microorganisms and promote the proliferation of hydrolytic microorganisms， which was conducive to the secretion of hydrolytic enzymes. As a result， the co-fermentation system can efficiently convert WAS and SMS into VFAs， which provides a theoretical reference for the resource recovery from WAS and SMS.

• Key words：waste sludge  SMS  pretreatment  anaerobic fermentation  hydrolysis and acidification

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