• 关键词：互花米草入侵  铁氧化菌  多样性  群落结构  河口湿地

• 基金项目：国家自然科学基金（No.42077086）；福建省公益类科研院所基本科研专项（No.2018R1034-1）

• 曾庆淞
• 福建师范大学生命科学学院，福州 350117

• 陈钰
• 福建师范大学生命科学学院，福州 350117

• 郑毅
• 福建师范大学生命科学学院，福州 350117

• 林美芬
• 福建师范大学生命科学学院，福州 350117

• 范玲玉
• 福建师范大学生命科学学院，福州 350117

• 许旭萍
• 福建师范大学生命科学学院，福州 350117

• 陈桂香
• 福建师范大学地理研究所，福州 350007;福建师范大学湿润亚热带生态地理过程教育部重点实验室，福州 350007

• 王维奇
• 福建师范大学地理研究所，福州 350007;福建师范大学湿润亚热带生态地理过程教育部重点实验室，福州 350007

• 摘要：以闽江口秋茄湿地、芦苇湿地及漳江口白骨壤湿地作为研究对象，通过高通量测序技术对互花米草入侵湿地前、后土壤铁氧化菌群落结构及其多样性进行测定与分析，以此探讨互花米草入侵对河口湿地土壤铁氧化菌的影响.结果表明：①不同类型的河口湿地土壤中铁氧化菌数量对互花米草入侵后的反应不同，互花米草入侵闽江口秋茄湿地和芦苇湿地后，湿地土壤铁氧化菌数量均显著增加（p<0.05）；而入侵漳江口白骨壤湿地后，湿地土壤铁氧化菌数量显著降低（p<0.05）；②互花米草入侵使秋茄湿地铁氧化菌多样性提高，使白骨壤湿地和芦苇湿地 土壤铁氧化菌多样性降低；③变形菌门（Proteobacteria）铁氧化菌是湿地优势菌门，尤其是互花米草入侵秋茄湿地后，其变形菌门铁氧化菌的 相对丰度明显提高，而脱硫杆菌门（Desulfobacterota）铁氧化菌的相对丰度降低；④互花米草入侵后，秋茄湿地中优势菌地杆菌属（Geobacter） 铁氧化菌的丰度显著降低，而沙雷氏菌属（Sediminibacterium）的丰度显著提高；芦苇湿地中互花米草入侵后披毛菌属（Gallionella）的丰度显著提高；⑤盐度、pH、易氧化态碳、微生物生物量碳等环境因子是土壤铁氧化菌属群落结构主要影响因素.研究结果可为探究互花米草入侵对 微生物生态的影响提供重要的数据支持，进而为湿地土壤生物多样性的保护提供理论科学依据.

• Abstract：In order to explore the effect of Spartina alterniflora invasion on iron-oxidizing bacteria in estuarine wetland， Kandelia obovate wetland in Minjiang estuary， Avicennia marina wetland in Zhangzhou estuary and Phragmite australis wetland in Minjiang estuary were taken as the research objects and soil iron-oxidizing bacteria community structure before and after Spartina alterniflora invasion were analyzed. The results showed that： ① The response of soil iron-oxidizing bacteria number to Spartina alterniflora invasion was different in various estuarine wetlands. Soil iron-oxidizing bacteria was significantly increased after Spartina alterniflora invasion in Kandelia obovate and Phragmite australis wetlands of Minjiang estuary （p<0.05）， while soil iron-oxidizing bacteria was significantly decreased after Spartina alterniflora invasion in Avicennia marina wetland of Zhangjiang estuary （p<0.05）. ② Iron-oxidizing bacteria diversity was increased after Spartina alterniflora invasion in Kandelia obovate wetland， and was decreased in the Avicennia marina and Phragmite australis wetlands. ③ Proteobacteria iron-oxidizing bacteria was the dominant bacteria in the wetland. The relative abundance of Proteobacteria iron-oxidizing bacteria was significantly increased， however， Desulfobacterota iron-oxidizing bacteria was decreased after Spartina alterniflora invasion. ④ The abundance of Geobacter iron-oxidizing bacteria was significantly decreased， while Sediminibacterium was significantly increased after Spartina alterniflora invasion in Kandelia obovate wetland. On the other hand， the abundance of Gallionella in Phragmite australis wetland was significantly increased after Spartina alterniflora invasion. ⑤ The environmental factors， such as soil salinity， pH， labile soil organic carbon and soil microbial biomass carbon were the main factors affecting the structure of the soil iron-oxidizing bacteria. The results could provide important data support for exploring the impact of Spartina alterniflora invasion on microbial ecology， and further provide theoretical and scientific basis for wetland protection and restoration.

• Key words：Spartina alterniflora invasion  iron-oxidizing bacteria  diversity  community structure  estuarine wetland

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