• 关键词：微藻  响应面  固碳  产油  沼液培养

• 基金项目：四川省科技厅国际合作项目（No.2019YFH0133）；山西省气象局科研项目（No.SXKZDDW20217105）

• 杨豪
• 成都信息工程大学资源环境学院，成都 610225

• 信欣
• 成都信息工程大学资源环境学院，成都 610225;中-塞环境与能源“一带一路”联合实验室，成都 610225

• 曹惜霜
• 成都信息工程大学资源环境学院，成都 610225

• 刘洁
• 成都信息工程大学资源环境学院，成都 610225;中-塞环境与能源“一带一路”联合实验室，成都 610225

• 徐成华
• 成都信息工程大学资源环境学院，成都 610225;中-塞环境与能源“一带一路”联合实验室，成都 610225

• 刘建英
• 成都信息工程大学资源环境学院，成都 610225;中-塞环境与能源“一带一路”联合实验室，成都 610225

• 摘要：为了提高能源微藻Chlorella vulgaris的固碳产油性能和进一步降低其培养过程培养成本，本研究在单因素结果的基础上，通过响应面法对其进行了优化；并在优化后的条件下，探讨了采用15% CO₂联合沼液替代传统培养基BG-11培养Chlorella vulgaris的可行性.结果表明，通气速率、光照强度、温度过高或过低都不利于小球藻生长、固碳和产油.响应面优化后得出当通气速率为0.075 m³?m^-3·min^-1、温度为28.5 ℃、光照强度为4950 lx时，小球藻的固碳和产油效果最佳，其生物质产率、平均固碳速率和油脂产率分别为0.20 、0.367g·L^-1·d^-1和56.8 mg·L^-1·d^-1.利用50%沼液联合15% CO₂培养Chlorella vulgaris时，其平均固碳速率、油脂产率分别为0.3304 g·L^-1·d^-1和42.81 mg·L^-1·d^-1，此时沼液中氨氮、总磷、COD的利用率分别能够达到55.48%、41.95%和81.63%，沼液可有效替代BG-11培养基，可大幅度降低培养成本，实现废水和CO₂的资源化.

• Abstract：In order to improve the carbon fixation and lipid production performance of the microalgae Chlorella vulgaris and reduce its culture process and cultivation cost， in this study its culture process cultivation was further optimized by response surface methodology based on the single-factor results. And then， the feasibility of using 15% CO₂ combined with biogas slurry to replace the traditional medium BG-11 for the cultivation of Chlorella vulgaris was investigated. The results showed that gas flow rate， light intensity and excessively high or low temperature were not conducive for the biomass growth， carbon sequestration and oil accumulation of Chlorella vulgaris. The maximal carbon sequestration and oil production were obtained under the optimized conditions of the gas flow rate 0.075 m³?m^-3·min^-1， 28.5 ℃ with the light intensity of 4950 lx. The corresponding biomass yield， average carbon fixation rate and oil yield were 0.20 g·L^-1·d^-1，0.367 g·L^-1·d^-1 and 56.8 mg·L^-1·d^-1 respectively. When Chlorella vulgaris was cultured with 50% digestate combined with 15% CO₂， its average carbon fixation rate and oil yield were respectively 0.3304 g·L^-1·d^-1 and 42.81 mg·L^-1·d^-1， and the utilization rates of ammonia nitrogen， total phosphorus and COD in digestate were 55.48%， 41.95% and 81.63%. The biogas slurry can be used to replace part of the BG-11 medium， which can greatly reduce the cultivation cost and realize the resource utilization of wastewater and CO₂.

• Key words：microalgae  response surface  carbon fixation  lipid production  manure liquid digestate

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