• 关键词：Zoogloea resiniphila HJ1  Methylobacterium rhodesianum H13  复合强化菌群  挥发性有机物  矿化率

• 基金项目：国家自然科学基金青年项目（No.21906150）；浙江省重点研发计划（No.2020C03085）

• 凌文华
• 浙江巴陵恒逸己内酰胺有限责任公司，杭州 311228

• 郑江玲
• 绍兴市生态环境局新昌分局，绍兴 312500

• 李云飞
• 浙江天弈环境有限公司，杭州 310012

• 胡俊
• 浙江工业大学环境学院，杭州 310014

• 赵景开
• 浙江工业大学环境学院，杭州 310014

• 於建明
• 浙江工业大学环境学院，杭州 310014

• 摘要：挥发性有机物（VOCs）排放导致一系列环境问题，而生物法是一项绿色处理技术.本实验选取3种VOCs（甲苯、邻二甲苯、二氯甲烷）作为模型污染物，利用甲苯、邻二甲苯降解菌Zoogloea resiniphila HJ1（菌株HJ1）及二氯甲烷降解菌Methylobacterium rhodesianum H13（菌株H13）构建复合菌株，采用“专属菌+综合菌”模式以复合菌株和驯化污泥构建复合强化菌群.研究表明，复合菌株可完全降解400 mg·L^-1甲苯、256 mg·L^-1邻二甲苯、200 mg·L^-1二氯甲烷，矿化率分别为74.3%、61.1%、82.7%.复合菌株可完全降解480 mg·L^-1混合污染物（3种污染物浓度比为1∶1∶1），降解速率依次为甲苯>邻二甲苯>二氯甲烷.经过优化，菌株HJ1和菌株H13最佳配比被确定为1∶5.复合强化菌群不仅大幅提高了 邻二甲苯、二氯甲烷降解速率，还提高了混合污染物矿化率.甲苯、邻二甲苯的存在对二氯甲烷降解产生明显抑制效应，而低浓度二氯甲烷 （< 240 mg·L^-1）对甲苯、邻二甲苯降解无明显抑制作用.本研究为VOCs生物净化过程中菌群构建提供数据支撑.

• Abstract：The emission of volatile organic compounds （VOCs） results in a series of environmental problems， and biological method is considered as a green treatment technology. Three VOCs， toluene， o-xylene and dichloromethane， were selected as the model pollutants. The composite bacteria were constructed by Zoogloea Resiniphila HJ1 （strain HJ1） degrading toluene and o-xylene， and Methylobacterium Rhodesianum H13 （strain H13） degrading dichloromethane. The model of "exclusive bacteria + comprehensive bacteria" was applied to construct enhanced composite bacteria using the composite bacteria and acclimated sludge. The composite bacteria completely degraded 400 mg·L^-1 of toluene， 256 mg·L^-1 of o-xylene and 200 mg·L^-1 of dichloromethane with mineralization rates of 74.3%， 61.1% and 82.7%， respectively. The composite bacteria completely degraded 480 mg·L^-1 of mixed pollutants （the concentration ratio of the three pollutants was 1∶1∶1）， and the degradation rates were in the order of toluene > o-xylene > dichloromethane. The ratio of strain HJ1 to strain H13 was optimized to be 1∶5. The enhanced composite bacteria not only greatly increased the degradation rate of o-xylene and dichloromethane， but also increased the mineralization rate of mixed substrates. The presence of toluene and o-xylene obviously inhibited the degradation of dichloromethane， while dichloromethane with a low concentration （< 240 mg·L^-1） had no evident inhibition on the degradation of toluene and o-xylene. The study provides data support for the bacterial community construction during the biological purification of VOCs.

• Key words：Zoogloea resiniphila HJ1  Methylobacterium rhodesianum H13  enhanced composite bacteria  VOCs  mineralization

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