• 关键词：聚苯乙烯微塑料（PS）  纳米氧化锌（ZnONPs）  斑马鱼  生态冠复合物  赋存状态

• 基金项目：国家自然科学基金 （No.41977340）

• 罗宏威
• 广东工业大学环境科学与工程学院，广州 510006

• 杜青平
• 广东工业大学环境科学与工程学院，广州 510006

• 钟钻佳
• 广东工业大学环境科学与工程学院，广州 510006

• 梁泳芳
• 广东工业大学环境科学与工程学院，广州 510006

• 陈姗慧
• 广东工业大学环境科学与工程学院，广州 510006

• 许燕滨
• 广东工业大学环境科学与工程学院，广州 510006;广东工业大学分析测试中心，广州 510006

• 彭进平
• 广东工业大学轻工化工学院，广州 510006

• 摘要：水环境中的生物大分子会在水中残留的颗粒物表面形成生态冠，从而影响颗粒物的赋存状态和生态行为.本研究测定了纳米氧化锌（ZnONPs）和聚苯乙烯微塑料（PS）与斑马鱼体外生物大分子作用形成的生态冠复合物的形貌、组分、水动力学直径及Zeta电位等，以此探究微、纳米颗粒生态冠复合物的赋存状态与形成规律.结果表明，斑马鱼体外生物大分子主要含有类酪氨酸蛋白、类色氨酸蛋白、类富里酸、可溶解性生物副产物和类腐殖酸.扫描电镜观察发现，PS和ZnONPs表面部分或全部被生物大分子包裹，形成生态冠复合物.三维荧光光谱分析 发现，PS和ZnONPs对各类生物大分子亲和力不同.随着作用时间延长（3~24 h），在PS生态冠组分中类酪氨酸蛋白的占比逐渐增加，类腐殖酸的占比逐渐减少；ZnONPs生态冠中这两类生物大分子组分的变化趋势呈相反的变化趋势；这两种生态冠复合物的组分变化都呈现时间-效应关系.PS-生态冠复合物和ZnONPs-生态冠复合物的水动力学直径都随着作用时间延长而增加.然而这两种生态冠复合物的Zeta电位绝对值却呈现不同的时间-效应关系，PS-生态冠复合物的Zeta电位绝对值随着作用时间延长而增加，ZnONPs-生态冠复合物的Zeta电位绝对值随着 作用时间延长而减小.上述结果表明，微、纳米颗粒在水体中的赋存状态与其所处生物环境、生态冠组分、表面电荷、水动力学直径等具有密切相关性，这为进一步研究微、纳米颗粒物在水体中的生态效应提供了重要的研究思路和科学依据.

• Abstract：Biomolecules can form eco-corona on the surface of residual particles in aquatic environment， which might influence occurrence characteristics and ecological behaviors of particles.In this study， zinc oxide nanoparticles （ZnONPs） and polystyrene microplastics （PS） were used to interact with in vitro biomolecules of zebrafish in water， which produced eco-corona complexes.Morphological characterizations， eco-corona components， hydrodynamic diameters and Zeta potential values were measured to study occurrence characteristics and formation mechanisms of micro- and nano-sized particle eco-corona complexes.The results showed that main components of biomolecules surrounding zebrafish were tyrosine protein-like substances， tryptophan protein-like substances， fulvic acid-like substances， soluble biological byproducts and humic acid like substances.Eco-corona was formed on the surface of PS and ZnONPs which were completely and partially coated by biomolecules shown in scanning electron microscopy.PS and ZnONPs had different affinity with five different biomolecules through analyzing three-dimensional fluorescence spectrum.With the time increasing from 3 h to 24 h， the proportion of tyrosine protein-like substances in PS eco-corona was gradually increased while the proportion of humic acid like substances was decreased.The changes of two components in ZnONPs eco-corona were opposite to those in PS eco-corona.The component changes in PS eco-corona and ZnONPs eco-corona were shown time-dependent effects.Hydrodynamic diameters in both groups of PS eco-corona complex and ZnONPs eco-corona complex were increased with time.However， the Zeta potential absolute values of PS eco-corona complex and ZnONPs eco-corona complex were changed in a different way.The former was increased with time， while the latter was decreased.These results suggested that the occurrence characteristics of micro- and nano-sized particles in water were closely related to their biological environments， eco-corona components， surface charges and hydrodynamic diameters， which has provided important ideas and scientific proof for further study on the ecological effects of micro- and nano-sized particles in water.

• Key words：polystyrene microplastics（PS）  zinc oxide nanoparticles （ZnONPs）  zebrafish  eco-corona complex  occurrence characteristic

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