生物炭强化硫自养反硝化过程中亚硝酸盐的积累
- Biochar enhanced nitrite accumulation during sulfur autotrophic denitrification
- 摘要：硫自养反硝化(SADN)可为厌氧氨氧化(Anammox)提供亚硝酸盐(NO2--N).为了促进SADN过程的NO2--N积累,本实验以S0为电子供体,考察了污泥-煤矸石基生物炭(SCBC)对SADN中NO2--N积累率及抗冲击能力的影响.实验结果表明,投加SCBC使SADN的NO3--N还原速率由70.6(mg-N·L-1)·h-1提升至107.7(mg-N·L-1)·h-1,NO2--N还原速率由30.5(mg-N·L-1)·h-1提升至35.1(mg-N·L-1)·h-1,这使得NO2--N积累率由48.2%提升至63.0%.当遇到冲击负荷(300mg·L-1的NO3--N)时,SADN的还原性能下降,6d后逐步恢复并继续提高,不投加则需12d才能恢复.投加SCBC60天后,PN/PS和PN值分别为3.51和5.27 mg·gSS-1,高于不投加的2.36和3.44 mg·gSS-1.高通量测序均能检测到反硝化菌Thiobacillus、Sulfurimonas和Thermomonas,投加SCBC对反硝化菌起到富集作用,使其占比分别由40.0%、0.61%、0.03%上升为51.8%、2.71%、3.47%. 总之,添加SCBC为提高SADN的NO2--N积累提供了一个新方法,这也为污泥和煤矸石的资源化利用提供了一种新的途径.
- Abstract：Sulfur autotrophic denitrification (SADN) can provide NO2--N for anaerobic ammonia oxidation (Anammox). In order to promote the accumulation of NO2--N in SADN process, this study used S0 as the electron donor and investigated the effects of sludge/coal gangue-derived biochar (SCBC) on the NO2--N accumulation rate and shock resistance in SADN. The results illustrated that the addition of SCBC increased the NO3--N reduction rate in SADN from 70.6 (mg-N·L-1) h-1 to 107.7 (mg-N·L-1) h-1, and the NO2--N reduction rate increased from 30.5 (mg-N·L-1) h-1 to 35.1 (mg-N·L-1) h-1, resulting in an increase in the NO2--N accumulation rate from 48.2% to 63.0%. When subjected to a shock load (300mg·L-1 of NO3--N), the reduction performance of SADN decreased, gradually recovered after 6 days, and continued to increase, while it took 12 days to recover without the addition of SCBC. After 60 days, the PN/PS and PN values were 3.51 and 5.27 mg·gSS-1, respectively, higher than those without SCBC addition, which were 2.36 and 3.44 mg·gSS-1, respectively. The denitrifying bacteria Thiobacillus, Sulfurimonas, and Thermomonas were detected by high-throughput sequencing analysis. SCBC supplementation enhanced these bacteria, increasing their proportions from 40.0%, 0.61%, and 0.03% to 51.8%, 2.71%, and 3.47%, respectively. In conclusion, SCBC addition presents a new avenue for NO2--N accumulation in SADN treatment. It also provides a novel approach for sludge and coal gangue utilization.
