光助漂浮型铜钼双金属氮化碳复合材料活化PMS去除水中抗生素抗性菌与抗性基因的性能及机理
- Study on the performance and mechanism of ARB and ARGs removal in wastewater by Cu0-MoS2/C3N4-EG enhanced peroxymonosulfate activation process
- 基金项目：国家重点研发计划项目(2021YFC3201302)
- 摘要：本文通过光还原法制备了一种漂浮型Cu0和MoS2双金属氮化碳复合材料Cu0-MoS2/C3N4-EG,构建了可见光助Cu0-MoS2/C3N4-EG活化PMS体系(Vis/Cu0-MoS2/C3N4-EG/PMS),用于去除水中抗生素抗性菌(ARB)和抗性基因(ARGs)。灭菌实验结果表明,Vis/Cu0-MoS2/C3N4-EG/PMS体系在材料投加量为0.22 g?L-1,PMS浓度为1 g?L-1时,仅需0.5 min即可灭活全部TC-ARB(初始浓度为5.4×107 CFU?mL-1)。灭菌过程能够破坏细胞膜的结构,进而导致DNA片段泄露并受到ROS的攻击,使得ARGs的水平转移频率降低；淬灭实验结果表明,起主要作用的活性物质是.OH、.SO4-和1O2。Vis/Cu0-MoS2/C3N4-EG/PMS体系对实际污水处理厂二沉池出水消毒处理结果表明,其能够在2 min内完成高效消毒,并且大幅降低水中各类ARGs的绝对丰度。研究结果为TC-ARB及其ARGs污染的同步高效去除提供了技术支撑和理论依据。
- Abstract：In this study, a kind of floating copper-molybdenum bimetallic carbon nitride Cu0-MoS2/C3N4-EG was synthesized, a visible-light-assisted Cu0-MoS2/C3N4-EG activated PMS system (Vis/Cu0-MoS2/C3N4-EG/PMS) was constructed for the removal of ARB and ARGs in water. The results showed that the inactivation of all TC-ARB in this system achieved within only 0.5 min (the initial concentration was 5.4×107 CFU?mL-1) when catalyst dosage was 0.22 g?L-1, PMS dosage was 1 g?L-1. This system could destroy the cell membrane of TC-ARB in water, and the leaked DNA fragments was further attacked by ROS which significantly reduce the horizontal transfer of ARGs. The quenching experiments showed that hydroxyl radical(.OH)、sulfate radical(.SO4-) and singlet oxygen (1O2) play a relatively large role in the system. For the secondary effluent of the actual sewage treatment plant, the system could be effectively sterilized within 2 minutes and greatly reduce the absolute abundance of various resistance genes in the water. This study provide technical support and theoretical basis for the removal of TC-ARB and ARGs.
