聚氯乙烯微塑料对厌氧消化的抑制及生物炭减缓机制
- Inhibition of anaerobic digestion by polyvinyl chloride microplastics and the slowing mechanism of biochar
- 摘要：微塑料(MPs)在城市污水处理厂中广泛存在,严重影响了厌氧消化(AD)过程.本研究深入探讨了聚氯乙烯微塑料(PVC-MPs)对AD的影响,并评估了污泥煤矸石基生物炭(SCBC)缓解这种抑制的潜力.同时,通过分析微生物群落演变,揭示了其作用机制.结果显示,当PVC-MPs的浓度达到0.1g·(g·VS)-1时,对AD抑制效果最显著,化学需氧量(COD)的去除率和甲烷含量分别下降了17.5%和26.7%.然而,当SCBC添加量为1.0g·L-1时,其缓解抑制作用最为明显,COD去除率和甲烷含量分别提升了8.3%和118.8%,并且pH恢复至适宜微生物生长的范围,稳定在6.8至7.2之间.高通量测序技术揭示,SCBC能够富集甲烷鬃菌属、甲烷杆菌属、甲烷八叠球菌属、绿弯菌门、变形菌门、拟杆菌门的生长,相对丰度分别提升了19.0%、29.3%、18.9%、76.1%、21.9%、10.8%.综上所述,SCBC为缓解微塑料在AD过程中造成的抑制提供了一种创新方法,同时也为污泥和煤矸石的资源化利用开辟了一种新途径.
- Abstract：Microplastics (MPs) are widely present in municipal wastewater treatment plants, significantly affecting the anaerobic digestion (AD) process. This study investigated the inhibitory effects of polyvinyl chloride microplastics (PVC-MPs) on AD and evaluated the potential of sludge coal gangue-based biochar (SCBC) to mitigate these effects. Furthermore, the mechanism of microbial community evolution was elucidated through microbial community analysis. The results indicated that when the PVC-MPs concentration reached 0.1 g·(g·VS)?1, AD inhibition was most pronounced, with the chemical oxygen demand (COD) removal rate and methane content decreasing by 17.5% and 26.7%, respectively. However, when the SCBC dosage was 1.0 g·L?1, the mitigation effect was most significant, leading to an 8.3% increase in COD removal rate and a 118.8% rise in methane content. Additionally, the pH stabilized within a range favorable for microbial growth (6.8–7.2). High-throughput sequencing analysis revealed that SCBC facilitated the enrichment of Methanobrilla, Methanobacterium, Methanococci, Crutarobacteria, Proteobacteria, and Bacteroidetes by 19.0%, 29.3%, 18.9%, 76.1%, 21.9%, and 10.8%, respectively. In summary, SCBC provides an innovative strategy to alleviate microplastic-induced inhibition in the AD process while offering a novel approach for the resource utilization of sludge and coal gangue.
